基于纳米Au胶表面增强拉曼光谱对水中链霉素残留量的快速检测

发布时间：2018-03-23 09:48

本文选题：表面增强拉曼光谱(SERS)　切入点：硫酸链霉素(STR)　出处：《光电子·激光》2016年08期

【摘要】：为实现快速检测水中硫酸链霉素(STR)的残留,探索建立一种STR水溶液的表面增强拉曼光谱(SERS,surface enhanced Raman spectroscopy)检测方法。应用电磁炉加热以柠檬酸三钠还原氯金酸(HAucl4)制成的Au胶纳米颗粒作为表面增强基底,先后分析了Au胶纳米颗粒的吸收光谱、STR水溶液的SERS以及拉曼峰归属;然后确定了最佳的光谱仪功率、Au胶纳米颗粒的加入量和吸附时间;最后以1 031cm-1处拉曼峰作为特征峰建立STR水溶液的标定曲线,并对标定曲线的准确度和精确度进行验证。实验结果表明,当水中STR质量浓度范围为2.0~20.0mg/L时,1 031cm-1处的拉曼峰强度与STR水溶液的质量浓度有良好的线性关系,线性方程为Y=293.31 X+435.42,决定系数R2为0.933 7。真实值与预测值之间的R2为0.964 3,均方根误差(RMSEP)为1.3043mg/L,回收率为92.1~133.0%,相对标准偏差(RSD,n=5)为0.88~2.41%。
[Abstract]:In order to quickly detect streptomycin sulfate residues in water, A new method for the detection of STR aqueous solution by surface enhanced Raman spectroscopy (SERS surface enhanced Raman spectroscopy) was developed. Au colloidal nanoparticles prepared by using trisodium citrate (trisodium citrate) as the substrate for surface enhancement were prepared by electromagnet heating. The absorption spectra of au colloidal nanoparticles and the SERS and Raman peaks of the aqueous solution were analyzed successively, and the optimal addition amount and adsorption time of au colloidal nanoparticles were determined. Finally, the calibration curve of STR aqueous solution was established with the Raman peak at 1 031cm-1 as the characteristic peak, and the accuracy and accuracy of the calibration curve were verified. When the concentration of STR in water is in the range of 2.0~20.0mg/L, there is a good linear relationship between the intensity of Raman peak at 1 031cm-1 and the concentration of STR in aqueous solution. The linear equation is YC 293.31 X 435.42, the coefficient of determination R2 is 0.933 7.The R2 between the real value and the predicted value is 0.964 3, the RMS EPV is 1.3043 mg / L, the recovery is 92.1mg / L, and the relative standard deviation is 0.882.41.
【作者单位】：江西农业大学工学院生物光电及应用重点实验室;
【基金】：国家自然科学基金(31101295) 江西省科技厅对外科技合作计划(20132BDH80005);江西省科技厅科技支撑(2012BBG70058)资助项目
【分类号】：O657.37;X832

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