模式生物斑马鱼的脂肪酸组分分析

发布时间：2018-03-23 15:43

本文选题：斑马鱼　切入点：脂肪酸　出处：《中国食品学报》2016年12期

【摘要】：为进一步确定膳食多不饱和脂肪酸的添加提供依据,本文选取与人类基因高度相似的模式生物斑马鱼为研究对象,采用气质联用法分析斑马鱼不同生长时间(四月龄,六月龄和七月龄)和不同生理部位(大脑、心脏、肾脏、尾鳍、鱼腹肉以及整鱼)中的脂肪酸的含量与组成。分析结果表明:在斑马鱼中共鉴定出25种脂肪酸;除斑马鱼整鱼外,鱼腹肉中的脂肪酸种类最多,为20种;其它器官中的脂肪酸皆为10余种。在所有的组织中,棕榈酸(C_(16:0)),硬脂酸(C_(18:0))和油酸(C_(18:1))都是主要的脂肪酸,约占各组织器官总脂肪酸的50%以上;在整鱼与鱼腹肉中,不饱和脂肪酸的含量高于饱和脂肪酸,而在大脑、心脏、肾脏和尾鳍中,情况相反。对成熟斑马鱼大脑的不同部位(端脑、视顶盖和小脑)中的脂肪酸组成研究表明,各部分的脂肪酸的种类无显著性差异。
[Abstract]:In order to provide the basis for further determination of dietary polyunsaturated fatty acid supplementation, zebrafish, a model creature with high similarity to human genes, was selected as the research object, and the growth time (4 months old) of zebrafish was analyzed by GC-MS. The fatty acid content and composition in different physiological parts (brain, heart, kidney, caudal fin, fish abdomen and whole fish) were analyzed. The results showed that 25 kinds of fatty acids were identified in zebrafish. Except for the whole fish of zebrafish, there are 20 kinds of fatty acids in the belly meat of the fish, and more than 10 kinds of fatty acids in the other organs. In all tissues, palmitic acid is 16: 0, stearic acid is 18: 0) and oleic acid Con Cnn 18: 1) are the main fatty acids. About 50% of the total fatty acids in all tissues and organs; in whole fish and fish belly meat, unsaturated fatty acids are higher than saturated fatty acids, while in the brain, heart, kidney and caudal fin, The study of fatty acid composition in different parts of the mature zebrafish brain (telencephalon, optic tectum and cerebellum) showed that there was no significant difference in the types of fatty acids in different parts of the adult zebrafish brain.
【作者单位】：江南大学食品学院;江南大学医学院;
【基金】：国家自然科学基金青年项目(21505054) 江苏省自然科学基金青年项目(BK20150132)
【分类号】：TS254.7;O657.63

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