可用于3D肝细胞培养的甘草次酸修饰海藻酸钠凝胶球的制备

发布时间：2018-03-23 21:48

本文选题：微流体　切入点：海藻酸钠　出处：《高等学校化学学报》2017年02期

【摘要】：制备了叔胺改性甘草次酸[GA-N(CH3)2]修饰的海藻酸钠[ALG-GA-N(CH3)2],并在温敏性琼脂糖的辅助作用下,利用微流体技术获得了高通量、单分散且粒径可控的ALG-GA-N(CH3)2微凝胶.考察了Span 80含量、疏水配体取代度、样品浓度和水/油相流速对微液滴制备的影响.研究结果表明,叔胺基改性可显著改善甘草次酸的亲水性;在Span 80质量分数为2.0%,疏水配体取代度小于12%,样品浓度小于15mg/m L,水相流速为1.5 m L/h,油相流速为6 m L/h条件下,可获得高通量、单分散及粒径为200μm的适用于细胞包封培养的微凝胶球.同时提供了一种三维培养肝细胞的新方法,为其在组织工程中的应用奠定了基础.
[Abstract]:GA-N(CH3)2 modified sodium alginate [ALG-GA-N(CH3)2] modified by tertiary amine was prepared. The ALG-GA-N(CH3)2 microgel with high throughput, monodisperse and controllable particle size was obtained by microfluid technique with the help of thermo-sensitive agarose. The content of Span 80 was investigated. The effects of hydrophobic ligand substitution, sample concentration and flow rate of water / oil phase on the preparation of microdroplets were investigated. The results showed that tertiary amine modification could significantly improve the hydrophilicity of glycyrrhetinic acid. When the mass fraction of Span 80 is 2.0, the degree of substitution of hydrophobic ligand is less than 12, the concentration of sample is less than 15mg/m L, the flow rate of water phase is 1.5 mL / h, and the flow rate of oil phase is 6 mL / h, the high throughput can be obtained. The monodisperse and 200 渭 m diameter microgel spheres were suitable for cell encapsulation and culture. A new method of three-dimensional hepatocyte culture was provided, which laid a foundation for its application in tissue engineering.
【作者单位】：南开大学功能高分子教育部重点实验室高分子化学研究所;南开大学天津市化学化工协同创新中心;
【基金】：国家自然科学基金(批准号:51273095) 天津市自然科学基金青年项目(批准号:14JCQNJCO3500) 教育部长江学者创新团队发展计划项目(批准号:IRT1257) 国家基础科学人才培养基金(批准号:J1103306)资助~~
【分类号】：O648.17

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