离子色谱法测定婴幼儿配方米粉中硫氰酸钠

发布时间：2018-03-24 18:18

本文选题：离子色谱法　切入点：硫氰酸钠　出处：《中国酿造》2017年10期

【摘要】：建立了一种简单、快捷测定婴幼儿配方米粉中硫氰酸钠的离子色谱分析方法。样品用水提取后使用甲醇沉淀淀粉类杂质,离心后取上清液过On Guard RP柱净化后,经Ion Pac AS16离子色谱柱(含AG16保护住)分离,用KOH淋洗液自动发生器进行梯度淋洗,抑制器采用外加水模式,电导检测器检测。结果表明硫氰酸钠在0.010~10.0 mg/L范围内线性关系良好(相关系数R2为0.999 0),方法的检出限为0.27 mg/kg,定量限为0.9 mg/kg,回收率为80.5%~98.1%,日内精密度试验相对标准偏差(RSD)(n=5)为1.8%~4.7%,日间精密度试验RSD(n=5)5%。将该方法应用于20个样品的检测,其中5个样品检出硫氰酸钠,含量0.95~2.04 mg/kg。该方法具有净化效果好、定量准确、灵敏快速的特点,适用于婴幼儿配方米粉中硫氰酸钠的检测。
[Abstract]:A simple and rapid method for the determination of sodium thiocyanate in infant formula rice flour was established. The sample was extracted with water and the starch impurities were precipitated by methanol. The supernatant was purified by on Guard RP column after centrifugation. It was separated by Ion Pac AS16 ion chromatographic column (including AG16 protection). Gradient elution was carried out by KOH eluent automatic generator, and the inhibitor was in the mode of external water. The results showed that the linear range of sodium thiocyanate was 0.010 ~ 10.0 mg/L (R ~ 2 = 0.999), the detection limit of the method was 0.27 mg / kg, the quantitative limit was 0.9 mg / kg, the recovery rate was 80.5 mg / kg, and the relative standard deviation of intra-day precision test was 80.5 mg / kg. This method was applied to the detection of 20 samples. Sodium thiocyanate was detected in 5 samples, and the content of sodium thiocyanate was 0.95g / kg. The method has the advantages of good purification effect, accurate quantification, sensitivity and rapidity, and is suitable for the determination of sodium thiocyanate in infant formula rice flour.
【作者单位】：广州检验检测认证集团有限公司;
【分类号】：O657.7;TS216

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