基于多级孔二氧化硅的葡萄糖传感器研究

发布时间：2018-03-24 23:38

本文选题：多级孔二氧化硅　切入点：葡萄糖传感器　出处：《扬州大学》2016年硕士论文

【摘要】：第一部分基于微孔-大孔二氧化硅材料(HS)的葡萄糖传感器的制备及应用采用二元溶剂体系表面活性剂自组装法,在水／乙醇二元溶剂体系中,以九水偏硅酸钠为硅源,以阳离子表面活性剂十六烷基三甲基氯化铵为模板,制备出具有大孔和微孔的多级孔二氧化硅(HS)。并基于此材料构建葡萄糖传感器,探讨了该葡萄糖传感器的电化学性能以及对葡萄糖的检测应用。研究表明,该传感器对葡萄糖有较好的电化学响应、良好的重复性和稳定性,其线性范围为0.1-3.25 mM,最低检出限为0.05 mM (S/N=3),灵敏度为1.81 mA-M-1·-cm-2。第二部分基于氨基化多级介孔二氧化硅材料(HPSNs-NH2)的葡萄糖传感器的制备及应用采用多元溶剂体系表面活性剂自组装法,在水／乙醇／乙醚三元溶剂体系中,以硅酸四乙酯为硅源,以阳离子表面活性剂十六烷基三甲基溴化铵为模板剂,以3-氨丙基三乙氧基硅烷为硅烷偶联剂,制备出氨基化多级介孔二氧化硅(HPSNs-NH2)。并基于此材料构建葡萄糖传感器,进一步探讨该葡萄糖传感器的电化学性能以及对葡萄糖的检测应用。研究表明,该传感器对葡萄糖具有较好的电化学响应能力且具有良好的重复性和稳定性,对葡萄糖检测的线性范围为0.1-2.60 mM,最低检出限为0.05 mM (S/N=3),灵敏度为3.95mA·M-1·cm-2。第三部分基于固载金纳米颗粒的多级孔二氧化硅材料(HPSNs-Au)的葡萄糖传感器的制备及应用利用自组装-静电吸附法制备固载金纳米颗粒的多级孔二氧化硅(HPSNs-Au),并基于此材料构建葡萄糖传感器。制备的材料具有较大的比表面积和良好的生物相容性,能为葡萄糖氧化酶提供良好的微环境；固载的金纳米颗粒有利于提高电子给体与电子受体间的传递速率,增强酶膜的导电性。研究表明,该传感器对葡萄糖具有快速的电化学响应能力,对葡萄糖检测的线性范围为0.05-1.35 mM,最低检出限为0.01mM(S/N=3),灵敏度为19.65mA·M-1·cm-2,且具有较高的选择性以及良好的重复性和稳定性。
[Abstract]:The first part is about the preparation and application of glucose sensor based on microporous and macroporous silica (HS2). In the water / ethanol binary solvent system, sodium metasilicate is used as silicon source. Using cationic surfactant cetyltrimethylammonium chloride as template, multiporous silica with macropore and micropore was prepared. Glucose sensor was constructed based on this material. The electrochemical performance of the glucose sensor and its application to glucose detection are discussed. The results show that the sensor has good electrochemical response, good reproducibility and stability to glucose. The linear range is 0.1-3.25 mm, the lowest detection limit is 0.05mM / s / N ~ (3 +), and the sensitivity is 1.81 mA-M-1 -cm-2.The second part is the preparation and application of a glucose sensor based on amino multistage mesoporous silica (HPSNs-NH _ 2) using a multi-component solvent system surfactant self-assembly method. In water / ethanol / ether ternary solvent system, tetraethyl silicate was used as silicon source, cationic surfactant cetyltrimethylammonium bromide as template and 3-aminopropyltriethoxysilane as silane coupling agent. An aminated multistage mesoporous silica HPSNs-NH _ 2 was prepared. Based on this material, a glucose sensor was constructed. The electrochemical properties of the glucose sensor and its application to glucose detection were investigated. The sensor has good electrochemical response to glucose and has good repeatability and stability. The linear range for glucose detection is 0.1-2.60 mm, the minimum detection limit is 0.05mm / s / n ~ (3) and the sensitivity is 3.95mA M ~ (-1) cm ~ (-2). The third part is the preparation and application of a glucose sensor based on multilevel porous silica material (HPSNs-Au) supported gold nanoparticles. -Electrostatic adsorption method was used to prepare multiporous silica loaded with gold nanoparticles, and glucose sensors were constructed on the basis of this material. The prepared materials have a large specific surface area and good biocompatibility. It can provide a good microenvironment for glucose oxidase, and the immobilized gold nanoparticles can improve the transfer rate between electron donor and electron receptor and enhance the conductivity of enzyme membrane. The sensor has a rapid electrochemical response to glucose. The linear range for glucose detection is 0.05-1.35 mm, the lowest detection limit is 0.01 mm / s / n ~ (-1), the sensitivity is 19.65mA M-1 / cm ~ (-2), and the sensor has high selectivity, good repeatability and stability.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.1

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