阴离子交换树脂纯化三七总皂苷的液质联用分析

发布时间：2018-03-26 19:25

本文选题：三七总皂苷　切入点：阴离子交换树脂　出处：《科学技术与工程》2017年31期

【摘要】：对阴离子交换树脂纯化的三七总皂苷(PNS)进行定性和定量研究,采用液质联用技术结合皂苷标准品对纯化后的三七总皂苷中主要单体皂苷类成分进行指认,确定化学物质基础;并通过三重四级杆多反应检测模式,对这些单体皂苷进行定量分析,确定各皂苷所占比例及总皂苷的纯度。阴离子交换树脂纯化的三七总皂苷中主要含有11种皂苷:人参皂苷Rg1[(195.6±3.3)mg·g~(-1)]、人参皂苷Rg2[(58.1±1.1)mg·g~(-1)]、人参皂苷Re[(102.8±3.1)mg·g~(-1)]、人参皂苷Rb1[(105.4±2.2)mg·g~(-1)]、人参皂苷Rd[(85.8±1.1)mg·g~(-1)]、人参皂苷Rh1[(65.7±1.4)mg·g~(-1)]、20-O-Glucosylginsenoside-Rf[(28.0±1.3)mg·g~(-1)]、人参皂苷F1[(28.1±0.6)mg·g~(-1)]、人参皂苷F2[(31.3±0.9)mg·g~(-1)],三七皂苷R1[(118.2±2.7)mg·g~(-1)]和三七皂苷R2[(62.6±0.9)mg·g~(-1)],总皂苷纯度约为88.16%;并且通过质荷比和色谱保留行为推测含有三七皂苷K。利用阴离子交换树脂纯化的三七总皂苷成分明确、纯度较高,可以作为三七总皂苷药物和健康产品开发的原料使用。
[Abstract]:The purification of Panax notoginseng saponins (PNSs) by anion exchange resin was studied qualitatively and quantitatively. The main monomer saponins in the purified panax notoginseng saponins were identified by liquid / mass spectrometry combined with the standard saponins, and the chemical substance basis was determined. The quantitative analysis of these monosaponins was carried out by using the multi-reaction detection mode of triple four-step rod. To determine the proportion of saponins and the purity of total saponins. The total saponins of Panax notoginseng purified by anion exchange resin mainly contain 11 kinds of saponins: ginsenoside Rg1 [195.6 卤3.3)mg gng-1], ginsenoside Rg2 [58.1 卤1.1)mg gng-1], ginsenoside re [102.8 卤3.1)mg gangli-1], ginsenoside Rb1 [105.4 卤2.2)mg gng-1], ginsenoside Rd [85.8 卤1.1)mg goug-1], ginsenoside Rd [85.8 卤1.1)mg goug-1], ginsenoside Re, ginsenoside Rb1, ginsenoside Rb1, ginsenoside Rd, ginsenoside Rd. Rh1, F _ 1, F _ 2, F _ 2, F _ 2, F _ 2, F _ 2, F _ 2, R _ 1 [118.2 卤2.7)mg g ~ (-1)], R _ 2 and R ~ 2, respectively, with a purity of 88.16g ~ (-1), were estimated to contain trisaponoside K by mass charge ratio and retention behavior. It can be used as raw material for the development of panax notoginseng saponins and health products.
【作者单位】：长春中医药大学;
【基金】：吉林省科技发展计划项目(20160201001YY)资助
【分类号】：O657.63;R284

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