功能化量子点检测环境样品中汞离子及过氧化氢的研究

发布时间：2018-03-29 04:38

本文选题：量子点　切入点：功能化　出处：《苏州大学》2016年硕士论文

【摘要】：最近几年,荧光量子点之所以能成为科学研究领域中的热点之一,是因为其具有独特而优异的物理化学性质和光学特性。本论文在查阅大量文献的基础上,通过合成性能良好的CdSe/CdS量子点,使用不同化学试剂对量子点的表面进行修饰,从而制备了具有较高选择性的量子点荧光探针,并将其用于检测重金属离子Hg2+和无机小分子H2O2的测定。本论文共分为三章:第一章:阐述了量子点的基本原理、发光特性、合成方法、表面修饰以及其在化学分析、生物医药领域的应用。第二章:通过有机相法合成了单分散性良好的CdSe/CdS量子点,采用巯基乙酸将量子点转移至水相。用硫脲(TU)对量子点表面进行功能化修饰,利用其与汞离子的螯合作用可以使量子点的荧光发生猝灭,在此基础上,建立了一种新方法用于测定Hg2+。对实验条件进行了优化,并探讨了量子点荧光猝灭的机理。所建立的分析方法的线性范围为1-300μg L-1,相关系数为0.9989,检测限达到0.56μg L-1。该方法用于检测实际样品中的汞离子,结果令人满意。第三章:采用还原型谷胱甘肽(GSH)对CdSe/CdS量子点进行功能化修饰,量子点的荧光发生猝灭,利用过氧化氢的氧化性可以将GSH氧化为GSSG,使得量子点的荧光强度恢复,基于此建立了测定H2O2的新方法,在最佳的实验条件下,该功能化量子点检测H2O2的线性范围为0.2-80μmol L-1,相关系数为0.9979,并尝试应用于实际样品的检测。
[Abstract]:In recent years, fluorescent quantum dots have become one of the hotspots in the field of scientific research because of their unique and excellent physical and chemical properties and optical properties. By synthesizing CdSe/CdS quantum dots with good properties and modifying the surface of quantum dots with different chemical reagents, a highly selective quantum dot fluorescence probe was prepared. This paper is divided into three chapters: chapter 1: the basic principle of quantum dots, luminescent properties, synthesis methods, surface modification and chemical analysis. Applications in Biomedicine. Chapter 2: monodisperse CdSe/CdS QDs were synthesized by organic phase method. The QDs were transferred to aqueous phase by thioglycolic acid. The surface of the QDs was functionalized by thiourea Tu. The fluorescence of quantum dots can be quenched by the chelation of mercury ion. A new method for the determination of Hg2 was established. The experimental conditions were optimized. The mechanism of fluorescence quenching of quantum dots was discussed. The linear range of the method was 1-300 渭 g / L ~ (-1), the correlation coefficient was 0.9989, and the detection limit was 0.56 渭 g / L ~ (-1). The method was used to detect mercury ions in practical samples. The results were satisfactory. In chapter 3, the CdSe/CdS QDs were functionalized by reduced glutathione glutathione (GSH), the fluorescence of QDs was quenched, the GSH could be oxidized to GSSGs by the oxidation of hydrogen peroxide, and the fluorescence intensity of QDs could be restored. Based on this, a new method for the determination of H2O2 was established. Under the optimum experimental conditions, the linear range of the functionalized QDs for detecting H2O2 was 0.2-80 渭 mol L-1 and the correlation coefficient was 0.9979. The method was applied to the detection of real samples.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：X830;O657.3

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