拉曼光谱中荧光抑制技术的研究新进展综述

发布时间：2018-03-30 12:21

本文选题：光谱分析　切入点：拉曼　出处：《光谱学与光谱分析》2017年07期

【摘要】：拉曼光谱技术反映了物质的结构特征,可用于分析有机或者无机样品的化学组分。但由于某些被测物的荧光背景远远强于拉曼信号,这些物质的拉曼光谱测量有时十分困难,这限制了拉曼光谱的广泛应用。因此有必要在拉曼检测中对荧光采取抑制措施以准确获取高信噪比的拉曼光谱指纹信息。近些年来,很多的相关研究探讨及发展了多种荧光抑制的新方法。在目前的科研活动中,常用的技术有表面增强拉曼光谱技术、傅里叶变换拉曼光谱技术、共焦显微拉曼光谱技术和高温拉曼光谱技术等。这些技术解决了拉曼光谱早期存在的一些问题,如荧光干扰、灵敏度低等,极大地扩展了拉曼光谱技术在各个领域的应用。而这些新方法可大致归类为物理/化学方法,基于光学性质不同衍生的方法,计算处理方法和其他方法。文章概括性的介绍了上述方法的理论、实现方式,并分析比较了各自的特点。
[Abstract]:Raman spectroscopy can be used to analyze the chemical components of organic or inorganic samples.However, because the fluorescence background of some substances is much stronger than the Raman signal, it is very difficult to measure the Raman spectrum of these substances, which limits the wide application of Raman spectroscopy.Therefore, it is necessary to suppress the fluorescence in Raman detection in order to obtain the high SNR Raman fingerprint information accurately.In recent years, many related studies have explored and developed a variety of new methods of fluorescence suppression.In the current research activities, the commonly used techniques include surface-enhanced Raman spectroscopy, Fourier transform Raman spectroscopy, confocal micro-Raman spectroscopy and high-temperature Raman spectroscopy.These techniques have solved some problems in Raman spectroscopy, such as fluorescence interference, low sensitivity and so on, which greatly expand the application of Raman spectroscopy in various fields.These new methods can be broadly classified as physical / chemical methods, calculation methods and other methods based on the different derivation of optical properties.This paper briefly introduces the theory and realization of the above methods, and analyzes and compares their characteristics.
【作者单位】：中国食品药品检定研究院;中国药科大学;
【基金】：国家科技支撑计划课题(2012BAK08B02)资助
【分类号】：O657.37

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