槲皮素作为荧光探针对氟离子的识别作用

发布时间：2018-03-30 18:08

本文选题：槲皮素　切入点：荧光探针　出处：《光谱学与光谱分析》2016年11期

【摘要】：槲皮素为天然黄酮类化合物,广泛存在于植物的根、茎、叶、花和果实中。槲皮素作为荧光探针检测氟离子不仅具有较好的选择性和灵敏度,而且与合成的荧光探针比,还具有来源广、环保、无毒等优点。实验将不同阴离子(F~-,Cl~-,Br~-,I~-,ClO_4~-,H_2PO_4~-)分别加入到槲皮素的二甲基亚砜(DMSO)溶液中,考查槲皮素溶液的荧光强度变化。实验发现当加入氟离子后,槲皮素在500nm处的荧光发射峰的强度降低,发生荧光猝灭,且其猝灭程度随着氟离子浓度的增大而改变,即荧光强度随着氟离子浓度的增大而减小,并呈线性变化。而其他阴离子的加入对槲皮素和槲皮素-氟离子体系的荧光发射强度影响不大,说明其他阴离子不影响槲皮素对氟离子的识别,显示了槲皮素对氟离子具有较好的选择性。由荧光滴定光谱和荧光滴定曲线得到槲皮素对氟离子的滴定方程为:y=-13.36x+173.4,线性关系为R2=0.991,线性范围为1.0×10~(-6)~8.0×10~(-6)mol·L~(-1),最低检测限为1.0×10-7 mol·L~(-1),表明槲皮素对氟离子的识别具有较高的灵敏度。进一步实验表明槲皮素识别氟离子的机理可能是氟离子的加入破坏了溶液体系的氢键,改变了槲皮素分子的共轭状态,发生分子内电荷转移,促使槲皮素荧光猝灭。用该法成功检测了样品中微量氟离子,回收率为100.67%~102.44%,精确度较好,测定结果稳定。
[Abstract]:Quercetin, a natural flavonoid, is widely found in the roots, stems, leaves, flowers and fruits of plants. Quercetin as a fluorescent probe not only has good selectivity and sensitivity, but also has better sensitivity than the synthetic fluorescent probe. It also has the advantages of wide source, environmental protection, non-toxicity and so on. In the experiment, different anionic anions were added to the dimethyl sulfoxide dimethyl sulfoxide (DMSOO) solution, and the fluorescence intensity of the quercetin solution was examined. It was found that the fluorescence intensity of the quercetin solution was changed with the addition of fluorine ion, and the different anions were added to the dimethyl sulfoxide dimethyl sulfoxide dimethyl sulfoxide (DMSO) solution. The intensity of fluorescence emission peak of quercetin at 500nm decreases, fluorescence quenching occurs, and the quenching degree changes with the increase of fluoride concentration, that is, the fluorescence intensity decreases with the increase of fluoride concentration. The addition of other anions had little effect on the fluorescence emission intensity of quercetin and quercetin-fluorine ion system, indicating that other anions did not affect the recognition of fluorine ions by quercetin. The results show that quercetin has good selectivity for fluorine ion, the titration equation of quercetin for fluorine ion is: yyyz-13.36x 173.4, the linear relation is R2O0.991.The linear range is 1.0 脳 1010-7 mol / L ~ (-1), the detection limit is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1). The results show that quercetin has a high sensitivity to the recognition of fluorine ions. Further experiments show that the mechanism of quercetin recognition of fluorine ions may be that the addition of fluorine ions destroys the hydrogen bonds of the solution system. The conjugated state of quercetin molecule was changed and the intramolecular charge transfer occurred, which promoted the fluorescence quenching of quercetin. The method was used to determine trace fluorine ion in the sample successfully. The recovery rate was 100.677.The precision was good and the determination result was stable.
【作者单位】：南京林业大学化学工程学院;南京林业大学南方现代林业协同创新中心;南京林业大学理学院;南京林业大学现代分析测试中心;南京林业大学林学院;
【基金】：国家林业局林业公益性行业科研专项项目(20120460102) 江苏省第四期“333高层次人才培养工程”科研项目(BRA2012171) 国家科技支撑计划课题项目(2012BAD21B04) 南京林业大学优秀博士学位论文创新基金项目资助
【分类号】：O657.3

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