改进的QuEChERS气相色谱-串联质谱法测定草莓中21种杀菌剂残留

发布时间：2018-03-31 14:17

本文选题：QuEChERS　切入点：气相色谱-串联质谱　出处：《农药学学报》2017年01期

【摘要】：采用改进的Qu ECh ERS气相色谱-串联质谱法建立了检测草莓中21种杀菌剂(螺环菌胺、甲霜灵、四氟醚唑、酞菌酯、嘧菌环胺、戊菌唑、氟吡菌酰胺、氟菌唑、嘧菌胺、E-苯氧菌胺、抑霉唑、腈菌唑、醚菌酯、环氟菌胺、肟菌酯、丙环唑、环酰菌胺、氟环唑、咪唑菌酮、啶酰菌胺、嘧菌酯)残留的方法。样品经乙腈提取,采用N-丙基乙二胺(primary secondary amine,PSA)和石墨化碳黑(graphitized carbon blacks,GCB)作为分散固相萃取(dispersive solid phase extraction,DSPE)的吸附材料,净化液经氮气吹干、用V(正己烷):V(丙酮)=9:1混合溶液定容,采用气相色谱-串联质谱(gas chromatography-tandem mass spectrometry,GCMS/MS)测定。结果表明:在质量浓度0.005~0.2 mg/kg范围内,21种杀菌剂与对应的峰面积间呈良好的线性关系;在0.01、0.05和0.1 mg/kg 3个添加水平下,21种杀菌剂的平均回收率均在70%~122%之间,RSD20%。该方法适用于草莓中21种杀菌剂残留的快速、高效和准确分析。
[Abstract]:A modified qu ECh ERS gas chromatography-tandem mass spectrometry method was developed for the determination of 21 fungicides (spirocycline, methylalaxil, tetraflurazol, phthalic ester, pyridamine, pentomycazole, flupyrimamide, flumazol) in strawberry. Methods for residue of pyridamine E- phenoxifen, imidazole, nitrilazole, etheromycin, cyclofluramine, oxime ester, propanazole, cyclomide, fluocyrazole, imidazolone, pyridamine, pyridamole). The samples were extracted by acetonitrile. N- propylethylenediamine primary secondary aminesia (PSAs) and graphitized carbon biographized GCBs (GCBs) were used as adsorbents for dispersive solid phase extraction (DSPE). The purified solution was dried by nitrogen, and the volume of the purified solution was determined by using the mixed solution of acetone 9: 1 (acetone 9: 1). Gas chromatography-tandem mass spectrometry (GC-MS) was used to determine chromatography-tandem mass spectrometric mass. The results showed that there was a good linear relationship between 21 kinds of fungicides and the corresponding peak area in the range of 0.005 ~ 0.2 mg/kg. The average recoveries of 21 fungicides were between 70% and 122%. The method was suitable for rapid, efficient and accurate analysis of 21 fungicides residues in strawberry.
【作者单位】：四川省农业科学院分析测试中心/农业部农产品质量安全风险评估实验室(成都);
【分类号】：S481.8;S668.4;O657.63

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