纳米粘土矿物复合电极对嘌呤化合物的电化学同时分析

发布时间：2018-04-05 06:52

本文选题：电化学传感器　切入点：碳纳米材料　出处：《江西农业大学》2017年硕士论文

【摘要】：痛风是一种普遍存在的反复发作性炎性疾病,早在二十世纪中期我国就已经出现了此类病例。研究发现,人体内尿酸(UA)产生过多是引起痛风的直接原因,而人体血液和组织液中嘌呤含量的升高则是产生痛风的根本原因,因而开发一种快速、简便、高效的嘌呤化合物分析方法具有重要的研究意义和应用价值。与传统的检测方法相比,电化学传感分析由于具有灵敏性高、选择性好、仪器简单易操作、检测成本低及可在线分析等特点,成为分析方法的一个重要研究方向。本论文通过结合纳米粘土材料和碳纳米材料的优点,利用羧甲基纤维素钠(CMC)良好的亲水性和粘附性,制备了水可加工性的、经济环保的嘌呤化合物纳米复合物传感器;围绕所构筑传感器的选择性、灵敏度、稳定性和实用性等特征展开了相应的研究,并成功应用于实际样品中嘌呤化合物的检测。具体结论如下:(1)通过选择强吸附性的埃落石纳米管(HNTs)和具有较好导电能力和催化能力的羧基功能化多壁碳纳米管(fMWCNTs)为主体材料,并借助于具有良好粘附性、亲水性的CMC溶液分散复合后,制备出具有良好电化学响应的CMC-HNTsfMWCNTs/GCE复合修饰电极。该复合修饰电极由于具有良好的稳定性、选择性和灵敏度,在最佳的实验条件下,将构建的电化学传感器用于UA、腺嘌呤(A)和鸟嘌呤(G)的同时电化学检测,线性响应范围分别为1 130μmol·L-1、0.05 9μmol·L-1和0.3 25μmol·L-1,检出限分别为0.5μmol·L-1、0.018μmol·L-1和0.019μmol·L-1。此外,CMC-HNTs-fMWCNTs修饰电极被成功的应用于人体血清和生菜样品中UA、A和G的同时检测。(2)在CMC的水溶液中,将吸附性强、比表面积大的凹凸棒石(ATP)与导电能力好的氮掺杂石墨烯(NG)进行分散复合,制备出能检测UA、黄嘌呤(XA)和次黄嘌呤(HX)的CMC-ATP-NG/GCE。该修饰电极具有良好的稳定性、选择性和灵敏度,对UA、XA和HX的线性范围分别为0.5 60μmol·L-1、0.2 20μmol·L-1和0.6 55μmol·L-1,检出限分别为0.13μmol·L-1、0.054μmol·L-1和0.39μmol·L-1。此外,CMC-ATP-NG修饰电极被成功的应用于水果和蔬菜样品中UA、XA和HX的同时检测。
[Abstract]:Gout is a common recurrent inflammatory disease, as early as the middle of the twentieth century, such cases have appeared in China.The study found that too much uric acid UAA is the direct cause of gout, and the increase of purine content in human blood and tissue fluid is the root cause of gout.The efficient method of purine compound analysis has important significance and application value.Compared with the traditional detection methods, electrochemical sensing analysis has become an important research direction because of its high sensitivity, good selectivity, simple and easy to operate instruments, low detection cost and on-line analysis.In this paper, by combining the advantages of nano-clay and carbon nanomaterials, a water-processable, economical and environmentally friendly purine compound sensor was prepared by utilizing the good hydrophilicity and adhesiveness of CMC-based sodium carboxymethyl cellulose.The selectivity, sensitivity, stability and practicability of the sensors were studied and successfully applied to the detection of purine compounds in practical samples.The specific conclusions are as follows: (1) by selecting HNTs with strong adsorbability and carboxyl functionalized multiwalled carbon nanotubes (FMWCNTs) with good conductivity and catalytic ability, and with the aid of good adhesion,The CMC-HNTsfMWCNTs/GCE composite modified electrode with good electrochemical response was prepared after dispersion of hydrophilic CMC solution.Due to its good stability, selectivity and sensitivity, the electrochemical sensor was used for simultaneous electrochemical detection of UAA and guanine guanine (G) under the best experimental conditions.The linear response ranges were 1: 130 渭 mol L -1 0. 05 渭 mol L -1 and 0 30 渭 mol L -1. The detection limits were 0. 5 渭 mol L -1 0. 018 渭 mol L -1 and 0.019 渭 mol L -1, respectively.In addition, CMC-HNTs-fMWCNTs modified electrode has been successfully used in the simultaneous detection of UAA and G in human serum and lettuce samples.High specific surface area attapulgite ATP-NGN / GCEs were prepared by dispersion and compounding with nitrogen-doped graphene (NGN) with high specific surface area. CMC-ATP-NGR / GCEs were prepared for the detection of UAA, xanthine (XA) and Hypoxanthine (HX).In addition, CMC-ATP-NG modified electrode was successfully used for simultaneous detection of UAXA and HX in fruit and vegetable samples.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1;R446.1;TS255.7

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