异硫氰酸苯酯柱前衍生反相高效液相色谱法测定复序橐吾中21种氨基酸的含量

发布时间：2018-04-05 10:04

本文选题：复序橐吾　切入点：氨基酸　出处：《中华中医药杂志》2016年12期

【摘要】：目的:测定复序橐吾中游离和水解氨基酸的含量。方法:以异硫氰酸苯酯(PITC)为柱前衍生试剂,用反相高效液相色谱法(RP-HPLC)测定复序橐吾中氨基酸的含量。结果:21种氨基酸的检测浓度线性范围为0.0022-2.1000μmol/L,精密度RSD在0.38%-1.05%之间;稳定性RSD在0.46%-1.28%之间;游离氨基酸和水解氨基酸的重复性RSD在1.20%-2.38%和0.86%-2.43%之间;游离氨基酸和水解氨基酸的加样平均回收率分别在97.89%-101.05%和98.04%-103.03%之间且RSD均小于4%,游离和水解氨基酸的含量分别为0.2307%和8.7943%。结论:复序橐吾中含有21种氨基酸,并确定了其含量。
[Abstract]:Objective: to determine the content of free and hydrolyzed amino acids in Ligularia complex.Methods: the content of amino acids in Ligularia complex was determined by reversed-phase high performance liquid chromatography (RP-HPLC) with phenylthiocyanate (PITC) as precolumn derivatization reagent.The average recoveries of free amino acids and hydrolyzed amino acids were 97.89-101.05% and 98.04% -103.03%, respectively, and RSD were less than 43.03%. The contents of free and hydrolyzed amino acids were 0.2307% and 8.7943, respectively.Conclusion: Ligularia polyphylla contains 21 amino acids and its content is determined.
【作者单位】：吉林农业大学中药材学院;吉林省食品药品审评中心;
【基金】：国家科技支撑计划项目(No.2011BAI03B01) 国家公益性行业科研专项(No.201303111) 科技部科技人员服务企业项目(No.2009GJB10031) 吉林省科技发展计划项(No.20130303094,No.20140204 013YY,No.20140311050YY,No.20150307012YY)~~
【分类号】：R284.1;O657.72

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