基于PDA修饰改性SERS基底的制备与研究

发布时间：2018-04-08 20:45

本文选题：SERS　切入点：柔性　出处：《江苏科技大学》2017年硕士论文

【摘要】：表面增强拉曼光谱(Surface enhanced Raman spectroscopy,SERS)自发现以来就备受关注,随着激光技术等技术发展,SERS技术的不断进步,该技术已经发展到用来定量检测的阶段,而且围绕该技术也产生了很多的新型基底模型。但是,SERS技术在普及性上还没有得到广泛的应用。论文中通过采用不同的制备方法得到了四种不同类型的基底,同时对基底各方面的的性能也进行了比较详细的表征与研究。主要的研究内容与结果如下:1.利用聚多巴胺(PDA)作为二级反应平台和原位还原剂,以银纳米颗粒(AgNPs)为基础制备Si/PDA/AgNPs基底。该基底表面均匀,有非常良好的信号重现性,各个特征峰的峰强的相对标准偏差(RSD)值都在10%以内,远低于20%的常规值。基底所能测量到的罗丹明6G(R6G)的最低浓度达到了10~(-8)M,增强因子(EF)达到了3.6×106。该方案在制备基底的过程中,充分利用了PDA的特性,制备过程中没有使用其它的还原剂,制备的步骤简单,反应过程绿色环保。解决了不少基底制备过程中的,还原复杂、有污染的弊端,在制备工艺的理念上更加的先进。2.实验在第一个体系的基础上,制备PDA/AgNPs/PDA型“三明治”结构基底。实验中PDA不但被当作二级反应平台,还被作为还原剂和保护膜使用。该“三明治”结构基底有很长的保存时间,在测试初期,由于PDA保护膜的覆盖,信号强度出现了一定的衰减,但衰减未达到一个数量级。“三明治”结构基底在后续测试中信号强度比普通基底更强更稳定,作为对照组的普通活性基底信号强度则随着时间的推移,呈现一种递减趋势。而且,“三明治”结构基底在六个月后极限时间测试时还有信号。作为对照,普通基底已无法检测到R6G的信号。在恶劣紫外光环境下的测试结果也表明:其性能即使在较长时间紫外光照射后基底依然有信号,相比与普通活性基底优势明显。3.论文中还进行了柔性SERS基底的开发,利用聚对苯二甲酸乙二醇酯(PET)和聚酯纤维布两种比较常见而且价格低廉的原材料进行基底的制作,制备的基底能测量到的R6G极限浓度也达到了10-8M,基底的重现性也很好,基底非常的均匀。而且基底在经过100次弯折后,在测试中信号强度几乎没有变化,说明柔性基底的抗性非常好。4.最后,方案设计核壳结构模型的SERS基底,与银溶胶类基底类似,实验中采用纳米银颗粒包裹二氧化硅以及用PDA包裹纳米银粒子两种方案,对两种基底的稳定性能进行研究。对比普通银胶基底,两种基底在性能保存时间上都有较大优势。由于在测试中在探针分子溶液的滴加上与平面或固体基底不同,所以表征中不再计算EF,而且由于采用核壳结构的目的就是对基底进行保护,从测试结果中可以看出,该模型完全达到了设计要求。论文中制备了四类活性基底,并且研究了基底的各种性能,发现基底在较长时间内都能保持性能的稳定,这使得它的应用前景变得更加多样。而且,由于其具有较长的“保质期”,所以其具有成为更加普及的消费品的潜力。
[Abstract]:Surface enhanced Raman spectroscopy (Surface enhanced Raman spectroscopy, SERS) has attracted much attention since it was discovered, along with the development of laser technology, the development of SERS technology, this technology has been used to develop quantitative detection stage, and around it also generate a new substrate model a lot. However, SERS technology has not been widely the application in the popularization of the thesis. By using different preparation methods and obtained four kinds of substrates of different types, and the performance of all aspects of the substrate were also characterized and studied in detail. The main research contents and results are as follows: 1. using polydopamine (PDA) as the two order reaction platform and in situ reduction agent, with silver nanoparticles (AgNPs) as the basis for preparing Si/PDA/AgNPs substrate. The substrate surface is uniform, the signal is very good reproducibility and the relative standard of each feature of the peak of strong The standard deviation (RSD) values are less than 10%, far lower than the conventional value of 20%. The substrate can be measured by the Luo Danming 6G (R6G) is the lowest concentration reached 10~ (-8) M, the enhancement factor (EF) reached 3.6 * 106. the preparation process of substrate in the system, make full use of the PDA the characteristics, preparation process without the use of other reducing agent, the preparation procedure is simple, green reaction process. To solve a lot of basement in the preparation process, reducing complexity, drawbacks of pollution, in the preparation process of the concept of more advanced.2. experiment based on the first system, system preparation of PDA/AgNPs/PDA type sandwich structure. The PDA substrate was not only as a two order reaction platform, also as a reducing agent and the use of the protective film. The "sandwich" structure of substrate has a long storage time, at the beginning of the test, the PDA protective film covering, there was a certain signal strength attenuation, But the attenuation did not reach a magnitude. The signal intensity of basal sandwich structure in the subsequent test is more stable than ordinary basement is stronger, as a common substrate signal strength in the control group with the passage of time, showing a decreasing trend. Moreover, the "basal sandwich structure" in the six months after the time limit test there was a signal. As a control, a common base has been unable to detect the R6G signal. In the harsh environment of the UV test results show that the performance even in a long time after ultraviolet irradiation is still a basal signal, compared with the ordinary active substrate have obvious advantages in the paper of.3. also developed flexible SERS substrate, using polyethylene terephthalate glycol ester (PET) and polyester fiber cloth made of two kinds of raw material is relatively common and low price of the substrate, the R6G limit of the prepared substrate can be measured by the concentration also At 10-8M, the basal reproducibility is also very good, very uniform. And the basal substrate after 100 after bending, in the test signal intensity is almost no change resistant flexible substrate is very good.4. finally, SERS base design core-shell structure model, similar to the silver sol substrate class, experiment the silver nanoparticles coated silica and silver nanoparticles coated by PDA two solution, to study stability of two kinds of substrate. Compared with common silver colloids, two kinds of substrate have great advantages in performance. Due to save time in the test on the probe molecule solutions adding on substrate and plane or solid different, so no longer in the characterization of EF is calculated, and the core-shell structure is to protect the substrate from the test results it can be seen that the model meets the design requirements. The preparation of four papers Type of substrate, and the study of the various properties of the substrate, the substrate that can maintain the performance in a relatively long period of stability, which makes its application become more diverse. But, because of its long shelf life, so it has become a more popular consumer potential.

【学位授予单位】：江苏科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.37

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