鱼组织中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量的超高效液相色谱法测定

发布时间：2018-04-08 23:29

本文选题：鱼组织　切入点：双去甲氧基姜黄素　出处：《分析测试学报》2017年02期

【摘要】：建立了黄颡鱼、团头鲂和草鱼血浆、肌肉、肝脏、肾脏中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素同时测定的超高效液相色谱紫外检测法(UPLC-UV)。样品经乙腈(含0.01%乙酸)提取,无水硫酸钠除水,正己烷去脂等样品处理,在ACQUIT UPLC BEH C18色谱柱上分离,428 nm波长处测定。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在0.01~5.00 mg/L浓度范围内呈良好的线性关系,相关系数(r2)分别为0.998 7,0.999 8和0.999 6。在空白鱼组织中进行0.025,0.05,0.50,1.00 mg/kg 4个水平的加标回收实验,3种待测组分的平均回收率为64.7%~102.2%,相对标准偏差为0.69%~10.8%。鱼组织中3种待测组分的检出限(LOD)均为0.010 mg/kg,定量下限(LOQ)均为0.025 mg/kg。应用该方法研究了姜黄素在团头鲂体内的药代动力学规律。
[Abstract]:A method for simultaneous determination of dimethoxycurcumin, desmethoxycurcumin and curcumin in plasma, muscle, liver and kidney of Pelteobagrus fulvidraco, Bream bream and grass carp was established.The samples were extracted by acetonitrile (containing 0.01% acetic acid), treated with anhydrous sodium sulfate, dewatered by n-hexane, and separated at 428nm on ACQUIT UPLC BEH C18 column.The linear range of dimethoxy curcumin, desmethoxycurcumin and curcumin was 0. 01 and 5. 00 mg/L, the correlation coefficient was 0. 0.998 and 0.999 6, respectively.The average recovery rate of the three components to be tested was 64.7% and 102.2%, and the relative standard deviation was 0.69 and 10.8, respectively, at the four levels of 0.025 ~ 0.05 ~ 0.50 ~ 1.00 mg/kg.The detection limit of the three components in fish tissues was 0.010 mg / kg and the lower limit of quantification was 0.025 mg / kg.The pharmacokinetics of curcumin in the body of Megalobrama bream was studied by this method.
【作者单位】：中国水产科学研究院长江水产研究所;淡水水产健康养殖湖北省协同创新中心;中国水产科学研究院;上海海洋大学水产与生命学院;
【基金】：现代农业技术体系专项资金(CARS-49) 中国水产科学研究院基本科研业务费资助(2016JBF0104) 公益性行业(农业)科研专项资助(201503108-CC-1)
【分类号】：S948;O657.72

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