姜黄素与金属离子的相互作用研究

发布时间：2018-04-09 01:09

本文选题：姜黄素　切入点：金属离子　出处：《石河子大学》2017年硕士论文

【摘要】：随着社会科技的发展以及人们对生物资源的深入研究,新型药物的研究重点逐渐转向天然产物的开发和利用。姜黄素是从姜科类植物中提取的一种具有广泛药用价值的生物成分,具有抗氧化、抗炎、抗菌、抗肿瘤等生物活性。Al~(3+)、Fe~(3+)、Cu~(2+)、Zn~(2+)是人体中重要的金属元素,对人体的各项生理及系统功能及新陈代谢中起着重要的作用。由于稀土元素在生物化学中的作用,促进了稀土配合物的发展应用。同时稀土元素具有特殊的光学、电学、磁性能,可作为生产制造出多种功能材料的基质和激活剂,在多领域有着良好的用途。稀土元素是亲氧的元素,稀土配合物的特征配位原子是氧,它们可以与含氧光电配体的β-二酮结构的姜黄素生成配合物。由于稀土与姜黄素生成的配合物具有优良的发光性能和药用作用,可作为发光材料、药用成分等被人们重视,并进行了广泛的研究。本实验研究了姜黄素与金属(Al、Fe、Cu、Zn)以及六种稀土元素(Y,Ce,Sm,Eu,,Tb,Er)的相互作用,合成了姜黄素金属配合物。利用紫外可见光谱和荧光光谱对合成配合物的实验条件诸如缓冲液的选择,pH值的影响,温度,反应时间,表面活性剂等进行了优化。并利用其体系荧光变化形成荧光探针,进行对部分药物和其它分子成分的定量测定和检测。通过对姜黄素金属配合物的荧光性质研究,以及在引入第三配体前后其体系荧光强度的变化,本实验发现了姜黄素-Cu~(2+)-异烟肼荧光体系检测异烟肼;姜黄素-Y~(3+)-氯诺昔康荧光体系检测氯诺昔康。通过与其它检测方法相比,本实验方法由于具有高准确性、高灵敏度等特点,在荧光分光光度法测定药物成分领域具有很好的应用前景。在研究β-二酮与稀土离子作用的实验后,对于同样具有β-二酮结构的氯诺昔康进行了研究,通过氯诺昔康-Eu~(3+)-溶菌酶三元配合物的合成及Eu~(3+)的荧光增强性质,建立了一种简单、灵敏的测定氯诺昔康荧光分析方法。其结果具有高灵敏度、高准确性及良好的选择性。这种荧光分光光度法测定氯诺昔康的方法可以很好的在医学领域进行应用
[Abstract]:With the development of social science and technology and the deep research on biological resources, the research focus of new drugs is gradually turning to the development and utilization of natural products.Curcumin is a kind of biological component with extensive medicinal value extracted from the family Curcumae. It has biological activities of anti-oxidation, anti-inflammation, anti-bacterial and anti-tumor. It is an important metal element in human body.It plays an important role in the physiological and systemic functions and metabolism of human body.The role of rare earth elements in biochemistry promotes the development and application of rare earth complexes.At the same time, rare earth elements have special optical, electrical and magnetic properties, which can be used as the matrix and activator for the production of various functional materials, and have good applications in many fields.Rare earth elements are oxyphilic elements, and the characteristic coordination atoms of rare earth complexes are oxygen, which can form complexes with curcumin with 尾 -diketone structure of optoelectronic ligands containing oxygen.Because the complexes of rare earth with curcumin have excellent luminescent properties and medicinal functions, they can be used as luminescent materials and medicinal components, and have been widely studied.In this paper, the interaction of curcumin with the metal Alniferoferin (Cuzn) and six rare earth elements (YCe, Sm, OU, TbCU) has been studied, and the curcumin metal complexes have been synthesized.UV-Vis spectra and fluorescence spectra were used to optimize the experimental conditions such as the pH value of buffer solution, temperature, reaction time, surfactant and so on.The fluorescence probe was formed by the fluorescence change of the system, and the quantitative determination and detection of some drugs and other molecular components were carried out.The fluorescence properties of curcumin metal complexes and the changes of fluorescence intensity of the system before and after the introduction of the third ligand were studied.Determination of lornoxicam by curcumin-yangzhuan3-lornoxicam fluorescence system.Compared with other detection methods, this method has a good application prospect in the field of fluorescence spectrophotometry because of its high accuracy and high sensitivity.After studying the interaction of 尾 -diketone with rare earth ions, the chlornoxicam with 尾 -diketone structure was studied, and the fluorescence enhancement properties of lornoxicam (Eu~(3) were studied by the synthesis of chlornoxicam -Euchloride-3-lysozyme ternary complex and the fluorescence enhancement of Lornoxicam.A simple and sensitive method for the determination of lornoxicam was established.The results show high sensitivity, high accuracy and good selectivity.The method for the determination of lornoxicam by fluorescence spectrophotometry can be well applied in the field of medicine.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O641.4;TQ460.1

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