基于磁性多壁碳纳米管在电化学分析多巴胺和槲皮素中的应用

发布时间：2018-04-09 05:17

本文选题：磁性多壁碳纳米管　切入点：β-环糊精　出处：《上海应用技术大学》2017年硕士论文

【摘要】：碳纳米管(CNTs)具有良好的导电性及大的比表面积,广泛用于修饰电极。但其溶解性差,难以分散,且用传统的滴涂法或者直接混合法修饰的电极物理稳定性差。本课题成功合成了磁性多壁碳纳米管(Fe304-MWCNTs),在外磁场辅助作用下,利用Fe304的超顺磁性可以提供一种将CNTs简单方便地修饰在丝网印刷电极(SPE)表面的方法。Fe304-MWCNTs修饰电极(Fe304-MWCNTs/SPE)能够检测痕量多巴胺(DA),线性范围为5 -180 μM,检出限为0.43 μM。同时,该修饰电极能够高选择性检测DA,而不受高浓度抗坏血酸(AA)的影响。此外,Fe304-MWCNTs/SPE用于进行人体血清中DA的加样回收实验,回收率在97.43 -102.94%之间,相对标准偏差(RSDs)低于2.27%。该工作提供了一种灵敏可靠检测DA的电化学方法,可用于判断一些与DA异常相关的神经细胞是否受到损伤。β-环糊精(β-CD)内腔疏水,外腔亲水的特殊结构使其能够作为分子识别原件,有选择性地将客体分子包含在其疏水内腔中形成主-客体包合物。用β-CD修饰电极,可以利用其能够形成主-客体包合物这一性质,将客体吸附在修饰电极表面,增加客体浓度,增强电信号。本课题成功合成了 β-CD功能化磁性碳纳米管(Fe304@β-CD-MWCNTs),通过磁吸附的方法将其修饰在丝网印刷电极(Fe304@β-CD-MWCNTs/SPE)表面,用于检测槲皮素(QR)。QR检测线性范围为0.95—100 μM,检出限为0.084 μM。同时,该修饰电极用于进行实际样品中QR的加样回收实验,如人体血清、洋葱、橘子和绿茶,回收率为95.92- 102.34%,RSDs低于2.54%。该工作提供了一种灵敏可靠检测QR的电化学方法,可用于检测实际生物样品中的QR含量。
[Abstract]:Carbon nanotubes (CNTs) have good electrical conductivity and large specific surface area, so they are widely used in modified electrodes.However, its solubility is poor, it is difficult to disperse, and the electrode modified by traditional drip coating or direct mixing method has poor physical stability.In this paper, the magnetic multi-wall carbon nanotubes Fe304-MWCNTsO have been successfully synthesized under the assistance of external magnetic field.The superparamagnetism of Fe304 can provide a simple and convenient method to modify CNTs on the surface of screen printing electrode. Fe304-MWCNTs / SPE can be used to detect trace dopamine. The linear range is 5-180 渭 m and the detection limit is 0.43 渭 M.At the same time, the modified electrode can be highly selective detection of DA-without the influence of high concentration of ascorbic acid (AAA).In addition, Fe304-MWCNTs / SPE was used for the recovery of DA in human serum. The recovery rate was between 97.43% and 102.94%, and the relative standard deviation (RSDs) was lower than 2.27%.This work provides a sensitive and reliable electrochemical method for the detection of DA, which can be used to determine whether some nerve cells associated with DA abnormalities have been damaged.The special hydrophilic structure of the external cavity enables it to be recognized as the original molecule and selectively include the guest molecule in its hydrophobic inner cavity to form a host-guest inclusion compound.By using 尾 -CD modified electrode, the host and guest inclusion compound can be formed, the guest can be adsorbed on the surface of the modified electrode, the concentration of the guest can be increased, and the electric signal can be enhanced.In this paper, the 尾 -CD functionalized magnetic carbon nanotubes Fe304@ 尾 -CD-MWCNTsO were successfully synthesized and modified on the surface of the screen printing electrode Fe304 @ 尾 -CD-MCNTsP / SPE by magnetic adsorption. The linear range of detection for quercetin was 0.95-100 渭 M and the detection limit was 0.084 渭 M.At the same time, the modified electrode was used for sample recovery of QR in real samples, such as human serum, onion, orange and green tea. The recovery rate was 95.92-102.34% lower than 2.54%.This work provides a sensitive and reliable electrochemical method for the detection of QR in biological samples.
【学位授予单位】：上海应用技术大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.1;O657.1;TB383.1

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