超临界流体色谱与高效液相色谱分离手性化合物的比较

发布时间：2018-04-11 22:34

本文选题：超临界流体色谱 + 高效液相色谱　；参考：《色谱》2016年03期

【摘要】：超临界流体色谱(SFC)分离具有速度快、分离效率高、溶剂消耗少等优点,近年来在手性化合物的分离分析中得到诸多应用。本文对比研究了涂覆型多糖手性色谱柱在SFC和高效液相色谱(HPLC)上拆分24种手性化合物的差异。通过比较这些化合物在色谱柱上的保留时间和选择因子等发现多数化合物在SFC上的分离效率要高于其在HPLC上的分离效率,但HPLC对轴手性化合物的分离效率要优于SFC。SFC和HPLC的分离表现出一定的互补性,随着苯环侧链烷基的碳数增加,化合物在SFC上的保留逐渐增强,而在HPLC的保留却逐渐减弱。叶菌唑在使用SFC和HPLC分析时出现了洗脱顺序反转的现象。这些结果为SFC手性拆分提供了参考。
[Abstract]:Supercritical fluid chromatography (SFC) separation has many advantages, such as high speed, high separation efficiency and less solvent consumption. In recent years, it has been widely used in the separation and analysis of chiral compounds.In this paper, the separation of 24 chiral compounds on SFC and high performance liquid chromatography (HPLC) on coated polysaccharide chiral column was studied.By comparing the retention time and the selection factor of these compounds on the chromatographic column, it was found that the separation efficiency of most compounds on SFC was higher than that on HPLC.However, the separation efficiency of HPLC was better than that of SFC.SFC and HPLC. With the increase of the alkyl number of benzene ring, the retention of the compounds on SFC gradually increased, but the retention in HPLC gradually weakened.The reversal of elution sequence was observed in the use of SFC and HPLC analysis.These results provide a reference for SFC chiral resolution.
【作者单位】：华南师范大学化学与环境学院;广州研创生物技术发展有限公司;
【基金】：国家自然科学基金项目(21171059;21571070) 科技型中小企业技术创新基金项目(13C26214404534) 广东省科技计划项目(2012B010900043,2014A010101145) 广州市科技计划项目(2013J4400027,201508020093)~~
【分类号】：O652.63

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