分子印迹固相萃取-高效液相色谱法检测蜂王浆及其制品中的蜂王酸

发布时间：2018-04-15 19:23

本文选题：分子印迹固相萃取 + 高效液相色谱　；参考：《中国食品学报》2017年08期

【摘要】：目的:建立分子印迹固相萃取与高效液相色谱联用方法,检测蜂王浆及其制品中蜂王酸的含量。方法:采用分子印迹本体聚合法,经一系列优化,得到对蜂王酸具有特异识别能力和高吸附容量的分子印迹聚合物。将其作为固相萃取柱的填充材料,对蜂王浆样品中的蜂王酸进行分离和纯化,采用高效液相色谱检测。结果:被测样品用分子印迹固相萃取柱处理后的加标回收率为92.02%~103.28%,检出限(S/N=3)为0.94μg/L,线性范围10~1 000μg/L(R20.99),相对标准偏差(RSD)小于8.91%。结论:所建方法具有准确性和实用性,可用于蜂王浆及其制品中蜂王酸的分离纯化和检测。
[Abstract]:Objective: to establish a method for the determination of bee royal acid in royal jelly and its products by molecularly imprinted solid phase extraction and high performance liquid chromatography.Methods: the molecularly imprinted polymers with special recognition ability and high adsorption capacity were obtained by a series of optimization methods by molecular imprinting bulk polymerization.It was used as a filling material for solid phase extraction column to separate and purify bee royal acid from royal jelly samples and was detected by high performance liquid chromatography (HPLC).Results: the recoveries of the samples treated with molecularly imprinted solid-phase extraction column were 92.022 and 103.28, the detection limit was 0.94 渭 g / L, the linear range was 101000 渭 g / L, and the relative standard deviation (RSDs) was less than 8.91%.Conclusion: the method is accurate and practical, and can be used for the separation, purification and determination of bee royal acid in royal jelly and its products.
【作者单位】：食品营养与安全教育部重点实验室天津科技大学食品工程与生物技术学院;
【基金】：国家重点研发计划项目(2016YFD0400401)
【分类号】：O657.72;S896.3

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