壳聚糖基功能微胶囊的制备及性能表征

发布时间：2018-04-16 23:38

本文选题：叶酸 + 磁性　；参考：《吉林大学》2017年硕士论文

【摘要】：在过去的几十年中,智能药物递送系统的研究在生物医药领域中备受关注。智能药物输送系统作为非常有效的输送载体被广泛用于递送药物、基因、细胞和酶。智能给药系统不仅无毒、无免疫原性、生物相容性和生物降解性,而且具有药物靶向输送及受外界刺激(例如,pH值,氧化还原梯度、温度和酶)触发释放药物的特性,可以有效提高化疗疗效及避免对健康组织产生的严重副作用。本文旨在研究多功能壳聚糖基微胶囊的声化学制备及其性能,利用叶酸(FA)修饰的巯基化壳聚糖通过超声化学法成功地制备了新颖的壳聚糖基多刺激响应型智能微胶囊(MSRS-CS-MCs)。靶向分子FA及发红色荧光染料RITC被固定在微胶囊MSRS-CS-MCs壳上,油酸改性的磁性纳米粒子及作为疏水性药物模板的绿色荧光染料香豆素6(C6)分散于油相被同时装载于微胶囊内。考察了超声强度、超声时间及浓度比对微胶囊尺寸的影响。此外,研究了微胶囊的磁靶向性能、细胞选择性和还原响应性释放药物的性能。研究表明,微胶囊上修饰的叶酸分子和囊内装载的油性磁性纳米粒子赋予其良好的分子靶向性和磁靶向性;所得微胶囊的交联囊壳可以保护所装载的药物安全递送到靶点不泄漏;囊壳中的二硫键具有还原响应性,在谷胱甘肽还原作用下可以快速断裂,赋予壳聚糖基载药微胶囊还原刺激响应性药物释放功能。
[Abstract]:In the past few decades, the research of intelligent drug delivery system has attracted much attention in the field of biomedicine.Intelligent drug delivery systems are widely used to deliver drugs, genes, cells and enzymes as very effective carriers.Intelligent drug delivery systems are not only non-toxic, non-immunogenic, biocompatible and biodegradable, but also have the properties of targeted delivery of drugs and the release of drugs triggered by external stimuli (such as pH, redox gradient, temperature and enzymes).Can effectively improve the efficacy of chemotherapy and avoid serious side effects on healthy tissue.In this paper, the sonochemical preparation and properties of multifunctional chitosan based microcapsules were studied. A novel chitosan based intelligent microcapsule MSRS-CS-MCsO was successfully prepared by ultrasonic chemical method.The target molecule FA and the red fluorescent dye RITC were immobilized on the microcapsule MSRS-CS-MCs shell. The oleic acid-modified magnetic nanoparticles and the green fluorescent dye coumarin 6 C6, a hydrophobic drug template, were dispersed in the oil phase and loaded in the microcapsule simultaneously.The effects of ultrasonic intensity, ultrasonic time and concentration ratio on the size of microcapsules were investigated.In addition, the magnetic targeting properties, cell selectivity and reduction response of microcapsules were studied.The results show that the modified folic acid molecules and the oily magnetic nanoparticles loaded in the microcapsules have good molecular and magnetic targeting properties.The cross-linked capsule of the microcapsule can protect the drug loaded safely from leakage to the target. The disulfide bond in the capsule is reductive and can break rapidly under the action of glutathione reduction.Chitosan-based drug-loaded microcapsules were given the function of reducing and stimulating responsive drug release.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O636.1;TQ460.1

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