增强氯化血红素催化活性用于水胺硫磷比色检测研究

发布时间：2018-04-17 01:08

本文选题：氯化血红素 + 水胺硫磷　；参考：《分析化学》2017年04期

【摘要】：建立了一种基于增强氯化血红素(Hemin)过氧化物酶催化活性比色检测水胺硫磷的方法。在H_2O_2存在下,Hemin催化氧化底物3,3',5,5'-四甲基联苯胺(TMB),使其失去1个电子,导致反应体系由无色变为蓝绿色。水胺硫磷的加入可提高Hemin对底物亲和力,进一步增强其催化活性,使底物氧化失去2个电子,反应体系由蓝绿色变为黄色,且颜色变化程度与水胺硫磷浓度成正比。在最优条件下,本方法的动态检测范围为2~100μg/L,检出限为1.2μg/L(3σ)。其它有机磷农药对水胺硫磷检测无明显干扰,实际样品中水胺硫磷的加标回收率为93.0%~113.0%。本方法可应用于农产品中水胺硫磷残留的检测。
[Abstract]:A method based on enhanced heme heminase catalytic activity colorimetry for the determination of sulphos was developed.In the presence of H_2O_2, Hemin catalyzes the oxidation of the substrate (3 ~ 3 ~ 3 ~ 5 ~ (5) C ~ (-1)) -tetramethyl benzidine (TMB), resulting in the loss of one electron, which leads to the reaction system changing from colorless to blue-green.The addition of sulphos can improve the affinity of Hemin to the substrate, further enhance its catalytic activity, and make the substrate oxidation lose two electrons. The reaction system changes from blue green to yellow, and the color change is proportional to the concentration of sulphos.Under the optimal conditions, the dynamic detection range of the method is 2 渭 g / L and the detection limit is 1. 2 渭 g/L(3 蟽路L ~ (-1).Other organophosphorus pesticides had no obvious interference with the determination of isophosphorus. The recoveries of isocyanidophos in actual samples were 93. 0% and 113. 0%.The method can be applied to the determination of sulphos residues in agricultural products.
【作者单位】：贵州大学发酵工程与生物制药重点实验室;贵州大学酿酒与食品工程学院;
【基金】：国家自然科学基金(Nos.21565009,21205020) 贵州省科学技术基金(黔科合基础[2016]1403) 贵州省科技厅科技合作计划(黔科合LH字[2015]7682) 贵州省教育厅招标项目(黔教合KY字[2014]261) 贵州大学引进人才科研项目(贵大人基合字(2014)09号);贵州大学研究生创新基金(No.2016056)资助~~
【分类号】：TQ450.263;O657.3

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