一种新型葡聚糖基水凝胶敷料的制备及其性质

发布时间：2018-04-17 04:23

本文选题：水凝胶 + 敷料　；参考：《东华大学学报(自然科学版)》2017年06期

【摘要】：将葡聚糖季铵盐衍生物(Dex-GTMAC)与丁二酸酐(SA)分别按摩尔比1∶1和1∶2反应,得到了不同羧基化程度的葡聚糖基大分子Dex-GTMAC-SA(1∶1)和Dex-GTMAC-SA(1∶2),并将其与合成的2,2'-(乙烯二氧)双(乙胺)甲基丙烯酰胺(DA-MA)小分子单体合成可光照交联的葡聚糖基大分子Dex-GTMAC-SA-DAMA(1∶1)和Dex-GTMAC-SA-DAMA(1∶2),再通过原位光交联方法制备得到新型生物可降解水凝胶敷料.通过测试得到Dex-GTMAC-SA-DAMA(1∶1)和Dex-GTMAC-SA-DAMA(1∶2)水凝胶的溶胀率分别为711.7%和645.0%;采用质量浓度为0.3mg/mL的胰蛋白酶磷酸盐缓冲液降解这两种水凝胶,5d后水凝胶形态不复存在,变成白色粉末,失重率分别为28.3%和22.5%;通过紫外分光光度法测得载药水凝胶在磷酸盐缓冲液(PBS)中浸泡10d后基本达到释药平衡,药物累计释放量分别达到68.4%和57.9%;通过琼脂平皿扩散法证实,载药的水凝胶相比于不载药的水凝胶,前3d的抑菌圈更为明显,显示出更为优良的抗菌性;通过荧光光谱分析法,得到两种水凝胶的表面蛋白吸附量分别为0.0038和0.0017mg/cm2,具有良好的抗蛋白吸附能力.研究结果表明该水凝胶有望成为一种新型的生物可降解的抗菌水凝胶敷料.
[Abstract]:Dex-GTMAC- (Dex-GTMAC-), a quaternary ammonium salt derivative of dextran, reacted with SA at 1:1 and 1:2, respectively.Dex-GTMAC-SA1 (1: 1) and Dex-GTMAC-SA1: 2 (1: 1) and Dex-GTMAC-SA1: 2 (1: 1) and Dex-GTMAC-SA1: 2 were synthesized with different carboxylation degree of dextran-based macromolecule Dex-GTMAC-SA1: 2, and synthesized from Dex-GTMAC-SA1: 1: 1) and synthesized by Dex-GTMAC-SA1: 1: 1, Dex-GTMAC-SA1: 1: 1).Dex-GTMAC-SA-DAMA-1: 1) and Dex-GTMAC-SA-DAMA1: 2). A new biodegradable hydrogel dressing was prepared by in situ photocrosslinking.The swelling rates of Dex-GTMAC-SA-DAMAA 1: 1) and Dex-GTMAC-SA-DAMA-1: 2) hydrogels were 711.7% and 645.0%, respectively.The weightlessness rates were 28.3% and 22.5% respectively, the drug loaded hydrogels were soaked in phosphate buffer PBSfor 10 days, and the cumulative drug release reached 68.4% and 57.9% respectively, and the results were confirmed by Agar plate diffusion method.The bacteriostasis of the hydrogel loaded with the drug was more obvious than that of the hydrogel without the drug, and the bacteriostasis of the hydrogel was more obvious than that of the hydrogel without the drug in the first 3 days.The surface protein adsorption capacities of the two hydrogels were 0.0038 and 0.0017 mg / cm ~ 2, respectively.The results show that the hydrogel is expected to be a new biodegradable antibacterial hydrogel dressing.
【作者单位】：东华大学纺织学院;东华大学纺织面料技术教育部重点实验室;
【基金】：中央高校基础研究基金资助项目(16D110113) 浦江人才计划基金资助项目(14PJ1400300)
【分类号】：TQ427.26

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