三唑类杀菌剂金属配合物的合成，表征，生物活性及缓释性能研究

发布时间：2018-04-19 01:26

  本文选题：三唑类杀菌剂 + 金属配合物　； 参考：《西北大学》2017年硕士论文

【摘要】：三唑类杀菌剂由于高效、广谱、低毒的优良特性,使其在农业杀菌方面具有突出优势,但是广泛的使用使菌种已经对其产生了抗药性,因此,寻找高效的、高安全性的、环境友好的新型真菌类杀菌剂势在必行。近年来,一些研究者发现农药杀菌剂和过渡金属通过配位反应形成金属配合物,可以提高杀菌活性同时降低毒性,减小对环境的污染。基于上述优点,本文以烯效唑和多效唑为配体合成了 6种金属配合物,对其进行了一系列表征,确定了其结构,并研究了六种金属配合物的杀菌性能和缓释效果。研究内容主要包括:首先,以2:1的摩尔比将烯效唑(L1)和多效唑(L2)分别与过渡金属盐在室温条件下搅拌,反应得到了 6种金属配合物。利用元素分析以及红外光谱等方法得到其元素比例及官能团等信息,用X射线单晶衍射仪确定晶体结构。利用SHELXTL程序进行晶体结构解析,并分析其结构特征,H键连接方式以及三维堆积方式。其次,对合成的6种金属配合物进行了生物活性研究。采用菌丝的生长速率法,在实验中测定了配合物、配体以及金属盐对苹果干腐病菌,苹果轮纹病菌,小麦赤霉病菌,葡萄黑豆病菌的抗真菌活性,并通过抑菌率及EC50值两个指标来评估抗菌性能。实验结果表明:[CoL14Cl2]的抑菌效果在烯效唑的三种金属配合物中最显著,尤其是对葡萄黑痘病菌(Ⅳ)的杀菌活性达到烯效唑配体的11.67倍,对其他几种菌也有较高的杀菌活性。多效唑的三种金属配合物中,[CuL24Br2]对苹果轮纹病菌(Ⅱ)、小麦赤霉病菌(Ⅲ)、葡萄黑痘病菌(Ⅳ)的杀菌活性都是最好的。最后,利用高效液相色谱仪(HPLC)测定了 6种配合物的缓释性能。首先通过对不同浓度标准烯效唑(L1)和多效唑(L2)溶液的测定,得到2种配体的HPLC标准曲线。然后测定所合成的六种配合物置于水中不同时间段缓慢释放的配体峰面积,并且通过浓度和峰面积的关系进一步计算其缓释率,用所得数据绘制时间-释放率图,分析缓释性能。
[Abstract]:Because of its high efficiency, broad spectrum and low toxicity, triazole fungicides have outstanding advantages in the field of agricultural sterilization.Environmental friendly new fungicides are imperative.In recent years, some researchers have found that pesticide fungicides and transition metals form metal complexes through coordination reactions, which can improve bactericidal activity and reduce toxicity and environmental pollution.On the basis of the above advantages, six metal complexes were synthesized with Uniconazole and paclobutrazole as ligands. Their structures were characterized, and the bactericidal properties and slow-release effects of the six metal complexes were studied.The main contents are as follows: firstly, six kinds of metal complexes were obtained by stirring with transition metal salts at room temperature at 2:1 molar ratio of Uniconazole L _ 1) and paclobutrazole L _ 2.The element ratio and functional group were obtained by elemental analysis and infrared spectroscopy. The crystal structure was determined by X-ray single crystal diffractometer.The crystal structure was analyzed by SHELXTL program, and the structure characteristics of H bond and three dimensional stacking were analyzed.Secondly, the bioactivity of the six metal complexes was studied.The antifungal activities of complexes, ligands and metal salts against dry rot fungus, rotavirus, scab of wheat and black bean of grape were determined by the method of mycelium growth rate.Bacteriostatic rate and EC50 value were used to evaluate the antibacterial performance.The results showed that the bacteriostatic effect of [CoL14Cl2] was the most significant in the three metal complexes of Uniconazole, especially the bactericidal activity of acidazole (鈪，

本文编号：1770991