软骨细胞体外增殖去分化的拉曼光谱分析

发布时间：2018-04-19 17:41

本文选题：软骨细胞 + 去分化　；参考：《物理化学学报》2017年12期

【摘要】：基于显微拉曼光谱技术,对组织工程的软骨种子细胞在传代增殖过程中的去分化进行单细胞分析。首先,对体外单层培养的第1-4代(P1-P4)大鼠软骨细胞样本进行了单细胞拉曼光谱检测,由此识别出软骨细胞中各种碱基、糖基、氨基酸等主要物质分子结构的特征峰集合。随后,分析拉曼光谱中若干重点特征峰强度随细胞传代次数的变化,发现软骨细胞体外增殖过程中核酸(789、1094、1576 cm~(-1))含量降低、Ⅱ型胶原(特异组分为羟脯氨酸,1207 cm~(-1))和蛋白聚糖(特异组分为糖胺聚糖,1042、1063、1126、1160 cm~(-1))合成下降、脂质(1304 cm~(-1))及磷酸盐(957 cm~(-1))含量增加等分子水平变化,从而在活体单细胞层次初步揭示了去分化引起软骨细胞增殖变缓、分泌减弱、形态纤维化等现象的分子机制。
[Abstract]:The dedifferentiation of cartilage seed cells in tissue engineering was analyzed by single cell method based on microscopic Raman spectroscopy.Firstly, single cell Raman spectroscopy was used to detect the single cell Raman spectra of the 1-4 passage P1-P4 rat chondrocytes cultured in vitro, and the characteristic peaks of the molecular structures of the main substances, such as base, glycosyl, amino acid and so on, in the chondrocytes were identified.Then, the changes of the intensity of some key characteristic peaks in Raman spectroscopy with the cell passage times were analyzed. It was found that the content of nucleic acid 789 ~ 1094 ~ (4 +) ~ 1576 cm ~ (-1) was decreased during the proliferation of chondrocytes in vitro.Thus, the molecular mechanism of dedifferentiation induced chondrocyte proliferation, secretion weakening and morphological fibrosis was revealed at the single cell level in vivo.
【作者单位】：北京大学前沿交叉学科研究院;北京大学-清华大学生命科学联合中心;北京大学信息科学技术学院纳米器件物理与化学教育部重点实验室;
【基金】：国家自然科学基金(U1636110) 科技部“科技支撑计划”(2012BAF14B14) 北京大学“医学-信息”交叉研究种子基金(2014-MI-19)资助项目~~
【分类号】：O657.37;R318.08

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