HPLC法同时测定不同产地佛手中5个成分的含量

发布时间：2018-04-21 17:51

本文选题：佛手 + 柚皮苷　；参考：《药物分析杂志》2017年12期

【摘要】：目的:建立HPLC波长切换法同时测定佛手药材中柚皮苷、橙皮苷、6,7-二甲氧基香豆素、5,7-二甲氧基香豆素和佛手柑内酯的含量。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.1%醋酸水(B),二元梯度洗脱(0~15 min,18%A→26%A;15~18 min,26%A→49%A;18~25 min,49%A;25~28 min,49%A→100%A),流速1.0 m L·min-1,柱温30℃,波长切换(0~15 min,在283 nm波长下检测柚皮苷和橙皮苷;15~28 min,在320 nm波长下检测6,7-二甲氧基香豆素、5,7-二甲氧基香豆素和佛手柑内酯)。结果:柚皮苷、橙皮苷、6,7-二甲氧基香豆素、5,7-二甲氧基香豆素和佛手柑内酯的进样量分别在9.3~186.6μg(r=0.999 8)、40.6~812.8μg(r=0.999 8)、8.4~167.7μg(r=0.999 9)、80.6~1 612.8μg(r=0.999 9)和4.3~86.4μg(r=0.999 9)范围内呈良好的线性关系;平均回收率(n=6)分别为95.5%、98.9%、95.8%、100.2%、97.7%,RSD分别为3.0%、2.6%、1.8%、2.6%、3.0%。11批佛手样品中柚皮苷、橙皮苷、6,7-二甲氧基香豆素、5,7-二甲氧基香豆素和佛手柑内酯含量测定结果分别为0.019%~0.068%、0.028%~0.275%、0~0.075%、0.094%~0.501%、0.002~0.034%。结论:本法可用于佛手药材中柚皮苷、橙皮苷、6,7-二甲氧基香豆素、5,7-二甲氧基香豆素和佛手柑内酯的含量测定。
[Abstract]:Objective: to establish a HPLC wavelength switching method for the simultaneous determination of naringin, hesperidin, 6,7- two methoxy coumarin, 5,7- two methoxy coumarin and citrus INOLIDE in Chinese medicinal herbs. Methods: Diamonsil C_ (18) column (250 mm x 4.6 mm, 5 mu m), acetonitrile (A) -0.1% acetate (B), two element gradient elution (15) ~18 min, 26%A - 49%A; 18~25 min, 49%A; 25~28 min, 49%A - 100%A), flow rate 1 m. The column temperature is 30, wavelength switching (naringin and hesperidin at 283 wavelengths, two methoxy coumarin, two methoxy coumarin and citrus INOLIDE). Results: naringin, hesperidin, and two The intake of methoxy coumarin, 5,7- two methoxy coumarin and bergamot lactone were linear in the range of 9.3~186.6 mu g (r=0.999 8), 40.6~812.8 mu g (r=0.999 8), 8.4~167.7 u g (r=0.999 9), 80.6~1 612.8 micron g (9) and 9 (9), respectively, 95.5%, 98.9%, 95.8%, 100.2%, 97.7, respectively. %, RSD were 3%, 2.6%, 1.8%, 2.6%, and 3.0%.11 batches of Buddhist hand samples, naringin, hesperidin, 6,7- two methoxy coumarin, 5,7- two methoxy coumarin and bergamot lactate, respectively, were 0.019%~0.068%, 0.028%~0.275%, 0~0.075%, 0.094%~0.501%, 0.002 ~0.034%., respectively: this method can be used in naringin, hesperidin, 6,7 in medicinal herbs. - Determination of the content of two methoxy coumarin, 5,7- two methoxy coumarin and bergamot lactone.

【作者单位】：川北医学院药学院药物研究所;川北医学院附属医院药剂科;
【基金】：川北医学院2015年校级科研发展项目(CBY15-A-YB22) 川北医学院博士项目(201402)
【分类号】：O657.72;R284.1

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