光果甘草叶中性多糖结构表征及抗氧化活性研究

发布时间：2018-04-22 22:28

本文选题：光果甘草 + 多糖　；参考：《现代食品科技》2016年01期

【摘要】：本文以光果甘草叶为原料,提取得到水溶性多糖GC C,采用DEAE-Sepharose Fast Flow柱层析法对GC C进行分离纯化,得到一种中性多糖(GC I),两种酸性多糖(GC II和GC III),选择DPPH自由基清除能力、氧自由基吸收能力(ORAC)强的中性多糖GC I,对其进行结构特征分析。采用高效凝胶渗透色谱法(GPC)、红外光谱法(FT-IR)、PMP柱前衍生-高效液相色谱法(HPLC)、甲基化-气相色谱质谱法(GC-MS)测定GC I的分子量、单糖组成以及糖苷键连接方式。GC I含有两个分子量分布不同的多糖,它们的平均分子量分别为980 ku及20 ku;红外光谱中4000~500 cm-1波数范围内出现了多糖的特征峰;GC I由5种单糖组成,包括:甘露糖、鼠李糖、葡萄糖、半乳糖和阿拉伯糖;GC I中→2)-Araf-(1→、→6)-Galp-(1→和→6)-Glcp-(1→残基含量最高。
[Abstract]:In this paper, the water-soluble polysaccharide GC C was extracted from Glycyrrhiza uralensis leaves. GC C was separated and purified by DEAE-Sepharose Fast Flow column chromatography. A neutral polysaccharide, two acidic polysaccharides, GC II and GC II, were obtained. The free radical scavenging ability of DPPH was selected. Oxygen free radical absorbability (ORAC) is a strong neutral polysaccharide GC I, and its structural characteristics are analyzed. The molecular weight of GC I was determined by high performance gel permeation chromatography (HGPC), FT-IRN PMP precolumn derivatization and high performance liquid chromatography (HPLC). The molecular weight of GC I was determined by methylation gas chromatography-mass spectrometry (GC-MS). There are two polysaccharides with different molecular weight distribution, their average molecular weights are 980ku and 20ku. the characteristic peaks of polysaccharides appear in the wave number range of 4 000 ~ 500 cm-1 in infrared spectrum, GCI is composed of 5 monosaccharides. Among them, mannose, rhamnose, glucose, galactose and arabinose were the highest in 2)-Araf-(1, 6)-Galp-(1 and 6)-Glcp-(1 residues.
【作者单位】：华南理工大学轻工与食品学院;
【基金】：“十二五”国家科技支撑计划项目(2012BAD37B08-01)
【分类号】：O629.12

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