新加香薷饮标准汤剂的HPLC指纹图谱

发布时间：2018-04-24 15:05

本文选题：新加香薷饮 + HPLC指纹图谱　；参考：《国际药学研究杂志》2017年03期

【摘要】：目的建立新加香薷饮标准汤剂(简称汤剂)的HPLC指纹图谱,为新加香薷颗粒(简称颗粒)的质量控制提供鉴定方法。方法采用高效液相色谱法,色谱柱为Phenomenex Luna C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%H3PO4溶液为流动相进行梯度洗脱,检测波长为280 nm,检测时间为130 min。采用2012版《中药色谱指纹图谱相似度评价系统》软件,以绿原酸峰为参照峰,建立汤剂对照指纹图谱,并将其与5批颗粒的指纹图谱进行相似度评价。结果 5批颗粒的指纹图谱与新加香薷饮标准汤剂对照指纹图谱的相似度均0.90。结论该方法简单、稳定、重复性好,可用于辅助控制颗粒的质量。
[Abstract]:Objective To establish a new HPLC fingerprint of Moslae Moslae decoction standard decoction ( abbreviated as decoction ) , and provide an identification method for the quality control of new Elsholtzia Moslae granules . The method adopts high performance liquid chromatography . The chromatographic column is used for gradient elution with acetonitrile - 0.1 % H3PO4 solution as mobile phase , the detection wavelength is 280 nm , and the detection time is 130 min . The fingerprint of 5 batches of particles is similar to that of the traditional Chinese traditional Chinese medicine decoction standard decoction reference fingerprint . The method is simple , stable and good in repeatability , and can be used to control the quality of particles .

【作者单位】：河南中医学院;仲景宛西制药股份有限公司;
【基金】：国家重大新药创制“经典名方标准颗粒的研究”(2013zx09508-105)
【分类号】：R286.0;O657.72

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