吡喃酮型花色苷衍生物的制备、光谱特性及抗氧化活性研究

发布时间：2018-04-27 23:47

本文选题：吡喃酮型花色苷衍生物 + Oxovitisin　；参考：《光谱学与光谱分析》2017年07期

【摘要】：吡喃酮型花色苷衍生物是一类具有非氧摀离子结构和内酯型吡喃环结构的新型多酚类化合物。本研究以植物花色苷为原料,通过羧基吡喃花色苷形成及微氧化等两步反应法,结合柱色谱分离纯化技术,制备高纯度的吡喃酮花色苷衍生物A(Oxovitisin A)标准品;高效液相色谱-光电二极管阵列检测器-串联质谱法(HPLC-DAD-ESI-MS/MS)分析鉴定出纯化后反应产物Oxovitisin A的纯度达99%以上。利用荧光光谱仪、紫外可见光谱仪及超微弱化学发光光谱仪研究了Oxovitisin A及其前体物质花色苷(锦葵素-3-葡萄糖苷,Mv-3-gluc)与羧基吡喃花色苷A(Vitisin A))的光谱特性、色泽稳定性及抗氧化活性。结果表明:Oxovitisin A在440nm激发波长下有最大荧光峰,最大发射波长490nm,而具有氧摀离子结构的两种前体物质无荧光特性。紫外光谱结合LAB色泽空间表征参数显示Oxovitisin A在不同pH值条件下具有不同的结构稳定性和色泽稳定性。Mv-3-gluc,VitisinA和Oxovitisin A在不同体系中均表现出良好的抗氧化活性,清除超氧阴离子自由基的IC50值分别为71.4,30.7和19μg·mL-1(抗坏血酸28μg·mL-1),清除羟自由基的IC50值分别为1.68,3.524,2.854μg·mL-1(抗坏血酸8.441μg·mL-1),对双氧水清除率的IC50值分别为1.311,0.4098和0.288μg·mL-1(抗坏血酸3.265μg·mL-1),表明Oxovitisin A清除自由基和抗氧化的能力均高于反应前体物Mv-3-gluc和VitisinA及抗坏血酸。
[Abstract]:Pyranone anthocyanin derivatives are a new class of polyphenols with non-oxygen structure and lactone-type pyran ring structure. In this study, anthocyanin was used as raw material to prepare high purity A(Oxovitisin A standard product by carboxyl pyranoside formation and micro-oxidation, combined with column chromatography separation and purification technology. High performance liquid chromatography-photodiode array detector tandem mass spectrometry (HPLC-DAD-ESI-MS) analysis showed that the purity of purified product Oxovitisin A was over 99%. The spectroscopic properties of Oxovitisin A and its precursor, Mv-3-glucin and carboxypyranoside A(Vitisin A, were studied by fluorescence spectrometer, UV-Vis spectrometer and ultraweak chemiluminescence spectrometer. Color stability and antioxidant activity. The results show that the maximum fluorescence peak and the maximum emission wavelength are 490nmm. the two precursor substances with oxygen structure have no fluorescence characteristics at the excitation wavelength of 440nm. UV spectra combined with LAB color spatial characterization parameters showed that Oxovitisin A had different structural stability and color stability under different pH conditions. Mv-3-glucanine A and Oxovitisin A showed good antioxidant activity in different systems. The IC50 value of scavenging superoxide anion radical was 71.4 渭 g mL -1 and that of ascorbic acid 28 渭 g mL -1, the IC50 value of scavenging hydroxyl radical was 1.68 渭 g ml -1 (8.441 渭 g ml -1 IC50 value of ascorbic acid 8.441 渭 g ml -1 and IC50 value of 0.288 渭 g ml -1 with ascorbic acid 3.265 渭 g mL -1, respectively). The ability of base and antioxidation was higher than that of Mv-3-gluc, VitisinA and ascorbic acid.
【作者单位】：武汉轻工大学食品科学与工程学院湖北省农产品加工与转化重点实验室;
【基金】：国家自然科学基金项目(31371727) 国家国际科技合作项目(2014DFG32310) 湖北省教育厅科学技术研究重点项目(D20121803)资助
【分类号】：O626;O657

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