基于光谱分析的液体浓度检测系统的设计与应用

发布时间：2018-04-28 06:21

本文选题：液体 + 蜂蜜　；参考：《南京航空航天大学》2017年硕士论文

【摘要】：液体中组分含量的检测在实际应用中是一个急需解决的问题,近年来相关的研究已经成为热点。复杂混合溶液成分及其含量的检测涉及到食品、药物、农业、环境等领域,它不仅与相应产品的品质、生产效率息息相关,而且也关系到食品安全等重大问题。随着生活水平和社会需求的提高,检测技术的要求也越来越高,人们希望检测技术能够更为方便、快捷、准确并且低碳和绿色环保。液体组分检测技术作为新的检测技术而日益受到重视,并且逐步拓宽到建筑、结构等诸多应用领域。目前,用于检测混合液体成分浓度的主要方法有密度瓶法、比重法、折光率法、高效液相色谱法、拉曼光谱法等等。密度瓶法由于其对实验环境条件要求较高,实现现场检测难度较大;比重法仪器简单,操作方便,但是计算复杂,结果误差较大;高效液相色谱法测量精度高但是操作不便,成本较高;而拉曼光谱法由于容易受到拉曼散射面积、光学系统参数、荧光现象等因素干扰而造成曲线非线性。为此,设计和研制一种操作简便并可用于现场的液体浓度检测光电系统十分必要。当前研究表明,使用荧光法检测溶液浓度时,在相同溶液厚度情况下,荧光强度与浓度成正比例关系;而测量其吸光度时,浓度与以10为底电压倒数的对数值成线性关系,基于此,本研究首先对现阶段液体组分的荧光光谱、紫外-可见分光光度检测技术与发展现状做了概述;其次详细阐述了荧光光谱法和紫外-可见分光光度法的原理,基于荧光光谱法,紫外-可见分光光度法设计并研制了新型的液体浓度光电检测系统,根据实际需要,在样品池后使用光子计数检测器构成检测荧光系统;而采用光敏二极管模块、数字式电压表构成测量吸光度系统,最后以蜂蜜为对象,进行了应用研究,验证了该设计系统的可行性,设计的系统实现了浓度检测的目的。论文主要研究内容有:(1)通过实验确定了样品溶液的最佳激发波长,分析得到了其浓度与荧光峰值之间的规律,建立了样品浓度和荧光峰值之间的数学模型,分析实验结果得到其相关系数,说明了本文所设计系统的可行性,随后通过实验验证了该系统设计的合理与有效性。(2)设计了基于荧光测量的液体浓度光电检测系统,该系统以紫外LED为光源光子计数检测器为检测元件,可以通过对液体溶液的荧光检测实现液体浓度检测;还设计了基于紫外—可见分光光度法测量液体浓度检测系统,该系统主要由光敏二极管模块和电压表构成,可通过对液体的吸光度检测,实现液体浓度检测。(3)对设计的基于检测荧光的液体浓度光电检测系统进行调试,并以蜂蜜为研究对象进行应用研究,通过实验测量得到蜂蜜浓度与荧光峰值强度之间的关系,建立了它们之间的数学模型,通过相同实验条件验证该数学模型的合理性,结果表明相关系数为0.94549,实现了液体浓度检测的目的;(4)对设计的基于吸光度测量液体浓度检测系统,进行可行性验证与应用研究。测量液体浓度与吸光度之间的关系,以蜂蜜为样品建立了其浓度与以10为底电压倒数的对数值的数学模型,在相同实验条件下验证其有效性,相关系数为0.98929,结果表明,该系统用于液体浓度检测方法有效。本文设计的新型的液体浓度光电检测系统,可实现液体浓度检测,采用该系统对典型液体-蜂蜜进行了应用研究,结果表明,该系统对液体浓度检测是可行和快速有效的,与已有方法相比,不仅更加方便、快捷、准确,并且操作简单,价格低廉,可以用于现场测量,本文研究为液体浓度检测与分析提供了新的途径。
[Abstract]:The determination of component content in liquid is an urgent problem in practical application. In recent years, the related research has become a hot spot. The detection of the composition and content of complex mixed solution involves food, medicine, agriculture, environment and other fields. It is not only related to the quality of the corresponding products, the production efficiency is closely related, but also related to food safety. With the improvement of living standards and social needs, the requirements of testing technology are becoming higher and higher. People hope that detection technology can be more convenient, quick, accurate and low carbon and green. As a new detection technology, liquid component detection technology has been paid more and more attention, and it has gradually widened to building, structure and so on. At present, the main methods used to detect the concentration of mixed liquid components are density bottle method, specific gravity method, refractive index method, high performance liquid chromatography, Raman spectroscopy and so on. The density bottle method is difficult to realize field detection because of its high requirement on the experimental environment, and the weight method is simple and easy to operate, but the calculation is complicated, but the result is complex and the result is complicated. The high performance liquid chromatography (HPLC) has high precision but inconvenient operation and high cost, and Raman spectroscopy is easy to be nonlinear due to the interference of Raman scattering area, optical system parameters, fluorescence phenomena and other factors. Therefore, a kind of photoelectric system which is easy to operate and can be used in the field of liquid concentration detection is designed and developed. It is very necessary. The current study shows that the fluorescence intensity is proportional to the concentration in the same solution thickness when the concentration of the solution is measured by the fluorescence method, and the concentration is linearly related to the value of the inverse of the bottom voltage inversion of 10. Based on this, the fluorescence spectra of the liquid components at the present stage are first studied. The visible spectrophotometric detection technology and the development status are summarized. Secondly, the principle of fluorescence spectrometry and ultraviolet visible spectrophotometry is described in detail. Based on the fluorescence spectrometry and ultraviolet visible spectrophotometry, a new photoelectric detection system for liquid concentration is designed and developed. According to the actual needs, the photon counting detection is used after the sample pool. The measuring apparatus forms the fluorescence system, and the light diode module and the digital voltmeter constitute the measuring absorbance system. Finally, the application research is carried out with honey as the object. The feasibility of the design system is verified. The designed system realizes the purpose of concentration detection. The main contents of the paper are as follows: (1) the sample solution was determined by the experiment. The optimum excitation wavelength of the liquid is obtained. The law between the concentration and the peak value of the fluorescence is obtained. The mathematical model between the concentration of the sample and the peak value of the fluorescence is established. The correlation coefficient is obtained by the analysis of the experimental results. The feasibility of the design of the system is illustrated. Then the design of the system is proved to be reasonable and effective. (2) the design is designed. Based on the fluorescence measurement system of liquid concentration photoelectric detection system, the system uses ultraviolet LED as the light photon counting detector as the detection component, can detect the liquid concentration by the fluorescence detection of liquid solution, and designs a measurement system of liquid concentration based on ultraviolet visible spectrophotometric method. This system is mainly light sensitive diode. The composition of the module and voltmeter can detect the liquid concentration by detecting the absorbance of liquid. (3) debugging the designed photoelectric detection system based on the detection of fluorescence, and using honey as the research object, the relationship between the concentration of honey and the peak intensity of the fluorescence is obtained by experimental measurement, and it has been established. The mathematical model between them verified the rationality of the mathematical model through the same experimental conditions. The results showed that the correlation coefficient was 0.94549, and the purpose of measuring the liquid concentration was realized. (4) the feasibility and application of the designed liquid concentration measurement system based on absorbance measurement. The relationship between liquid concentration and absorbance was measured. The mathematical model of its concentration and the inverse number of 10 as the bottom voltage is set up with honey as the sample. The validity of the model is verified under the same experimental conditions and the correlation coefficient is 0.98929. The result shows that the system is effective for the detection of liquid concentration. The new liquid concentration photoelectric detection system designed in this paper can realize the liquid concentration detection, The application of this system is applied to the typical liquid honey. The results show that the system is feasible, fast and effective for the detection of liquid concentration. Compared with the existing methods, it is not only more convenient, fast and accurate, but also simple in operation and low in price. It can be used in the field measurement. This paper provides a new method for the detection and analysis of liquid concentration. Way.

【学位授予单位】：南京航空航天大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;S896.1

【参考文献】