应用基于LC-TOF-MS分析的质量亏损过滤方法筛选黄芪注射液中的皂苷类成分

发布时间：2018-05-01 08:01

本文选题：黄芪注射液 + 黄芪皂苷　；参考：《中国中药杂志》2017年04期

【摘要】：采用液相色谱与四极杆飞行时间质谱(LC-TOF-MS)联用技术分析黄芪注射液(HI)样品,获取正负离子模式下HI中化学成分的LC-TOF-MS分析数据。根据前期相关文献报道,建立黄芪皂苷类化合物的质量亏损过滤(MDF)方法,用于系统筛选HI中所含有的皂苷类成分。每个筛选化合物的存在需由不少于2个准分子离子进行确认。最后依据其母离子和子离子信息对所筛选的化合物进行初步鉴定。最终共从HI中筛选并初步鉴定出62个皂苷类化合物,其中15个为新发现的皂苷类化合物。鉴定结果提示,乙酰化、氢化、去氢化、甲氧基化和水化可能为HI中皂苷所涉及的主要转化反应。该研究丰富了黄芪化学物质基础研究内容,同时也表明基于LC-TOF-MS分析的MDF方法,是一种可行且有效的中药成分系统筛选工具。
[Abstract]:The liquid chromatography and quadrupole time-of-flight mass spectrometry (LC-TOF-MS) were used to analyze the samples of Astragalus membranaceus injection (Astragalus membranaceus). The LC-TOF-MS analysis data of the chemical constituents in HI were obtained under the positive and negative ion mode. In this paper, a mass deficit filtration (MDF) method of astragalus saponins was established, which was used to screen the saponins in HI systematically. The presence of each screening compound needs to be confirmed by no less than 2 excimer ions. Finally, the selected compounds were preliminarily identified based on the information of their parent ions and subions. A total of 62 saponins were screened and identified from HI, 15 of which were newly discovered saponins. The results suggested that acetylation, hydrogenation, dehydrogenation, methoxylation and hydration may be the major conversion reactions involved in the saponins in HI. This study enriches the basic research contents of chemical substances of Astragalus membranaceus, and also shows that the MDF method based on LC-TOF-MS analysis is a feasible and effective tool for the systematic screening of components of traditional Chinese medicine.
【作者单位】：南京中医药大学附属医院临床药理实验室;中国药科大学基础医学与临床药学院;南京中医药大学附属医院药学部;
【基金】：国家自然科学基金青年项目(81503300) 江苏省自然科学基金青年项目(BK20131035) 江苏省中医院高峰人才项目(y2014rc17)
【分类号】：R286.0;O657.63

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