纤维素的手性分离特性研究

发布时间：2018-05-02 15:25

本文选题：D + L-扁桃酸　；参考：《云南师范大学》2016年硕士论文

【摘要】：本论文主要工作有以下几个方面:1.简要介绍了手性及手性分离的意义、手性分离方法、手性拆分的现实意义及纤维素在手性分离中的应用。重点介绍了高效液相色谱手性固定相对手性化合物的拆分和膜分离技术。2.纤维素是天然的多糖,分子内含有大量的羟基和手性位点,以纤维素为膜材料,制备了纤维素膜,考察了纤维素浓度、原料液浓度、浸泡时间、浸泡温度等因素对膜吸附性能的影响。实验结果表明:在最优的实验条件下,该膜对D,L-扁桃酸表现出较好的手性吸附性能,e.e.%值达到48.9%。实验还制备了纤维素手性固相萃取剂,将其填充到固相萃取管中,对D,L-扁桃酸进行萃取研究,并对固相萃取条件进行优化。纤维素固相萃取剂对D,L-扁桃酸的萃取也具有一定的手性选择性。3.将纤维素溶解在DMAc-LiCl中形成铸膜液,将其涂覆在3-氨丙基三乙氧基硅胶上,制备了纤维素手性柱,采用不同体积比的正己烷/异丙醇、甲醇/水、纯甲醇、纯水作为流动相,用高效液相色谱拆分D,L-扁桃酸,得到一定的分离效果。将纤维素膜取代纤维素手性柱进行膜色谱研究,实验表明纤维素手性膜色谱对D,L-扁桃酸也有一定的手性识别能力。4.将制备的纤维素膜,分别以浓度差和压力差为膜过程驱动力,考察了纤维素浓度、原料液浓度、渗过时间和流速不同时,其对D,L-扁桃酸吸附效果的影响,在最优的实验条件下,该膜对D,L-扁桃酸的手性拆分的e.e.%值分别达到70.1%和20.9%。
[Abstract]:The main work of this paper is as follows: 1. The significance of chirality and chiral separation, the method of chiral separation, the practical significance of chiral separation and the application of cellulose in chiral separation were briefly introduced. The resolution and membrane separation techniques of chiral stationary relative chiral compounds by high performance liquid chromatography (HPLC). Cellulose is a natural polysaccharide with a large number of hydroxyl groups and chiral sites in the molecule. Cellulose membrane was prepared with cellulose as the membrane material. The concentration of cellulose, the concentration of raw material solution and the soaking time were investigated. The influence of soaking temperature on the adsorption performance of the membrane. The experimental results show that under the optimum experimental conditions, the chiral adsorption property of the film for Dnlmandelic acid is 48.9%. Cellulose chiral solid phase extractant was also prepared and filled in a solid phase extraction tube to study the extraction of DL- mandelic acid. The conditions of solid phase extraction were optimized. Cellulose solid phase extractant also has chiral selectivity. Cellulose was dissolved in DMAc-LiCl to form a casting solution and coated on 3-aminopropyl triethoxy silica gel. The chiral cellulose column was prepared by using n-hexane / isopropanol, methanol / water, pure methanol and pure water as mobile phase. Dichlor-mandelic acid was separated by high performance liquid chromatography (HPLC). Cellulose membrane instead of cellulose chiral column was studied by membrane chromatography. The experimental results showed that cellulose chiral membrane chromatography also had a certain chiral recognition ability to DnL- mandelic acid. The effects of different concentration of cellulose, concentration of raw material liquid, permeation time and flow rate on the adsorption of DL- mandelic acid were investigated by using the concentration difference and pressure difference as the driving force of the membrane process, respectively, under the optimum experimental conditions, the effect of the cellulose membrane on the adsorption of DL- mandelic acid was studied under the optimum experimental conditions. The chiral resolution value of the membrane was 70.1% and 20.9%, respectively.
【学位授予单位】：云南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O636.11

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