基于氧杂蒽和萘酰亚胺的新型荧光探针的合成及性能研究

发布时间：2018-05-03 15:31

本文选题：荧光探针 + 罗丹明B　；参考：《郑州大学》2016年硕士论文

【摘要】：氧杂蒽类染料(罗丹明、荧光素),当螺环结构存在时荧光很弱且溶液是无色的,但当以开环形式存在时,会产生很强的荧光,并且溶液颜色会发生显著的变化。萘酰亚胺类染料具备分子结构简单、热稳定性好、光稳定性强、发射波长适中且易于修饰等优点,因而也是一种非常理想的荧光载体。本文主要设计并合成了两个基于罗丹明B的荧光探针(L1-L2),两个基于荧光素的荧光探针(L3-L4)和一个基于萘酰亚胺的荧光探针(L5),进一步考察了其光谱性能和在细胞成像方面的应用。目标化合物(L1-L5)的结构均经过1H NMR、13C NMR、HRMS的确证。化合物L1和L2是Fe3+探针,能够在甲醇与水体系中选择性识别Fe3+;化合物L3是SO32-探针,化合物L4是N2H4探针,化合物L5是Cu2+探针。化合物(L1-L5)均成功应用于活细胞内荧光成像检测,表明它们在生物体系内具有潜在的应用价值。
[Abstract]:Oxygen heteranthracene dyes (Rhodamine, fluorescein), when the snail ring structure exists, the fluorescence is very weak and the solution is colorless, but when it exists in the open ring form, it will produce very strong fluorescence, and the color of the solution will change significantly. Naphthalimide dyes have the advantages of simple molecular structure, good thermal stability, strong light stability, moderate emission wavelength and easy modification, so they are also an ideal fluorescent carrier. In this paper, we designed and synthesized two fluorescent probes based on Rhodamine B, two fluorescent probes based on fluorescein, and a fluorescent probe based on naphthalene imide. The spectral properties and applications in cell imaging were further investigated. The structure of the target compound L1-L5 was confirmed by 1H NMR-13C NMR-HRMS. Compounds L1 and L2 are Fe3 probes, which can selectively recognize Fe3 in methanol and water systems. Compound L3 is SO32- probe, compound L4 is N2H4 probe, and compound L5 is Cu2 probe. Compounds L _ (1-L _ 5) have been successfully applied to the detection of fluorescence imaging in living cells, indicating that they have potential application value in biological systems.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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