穿心莲内酯高压液相色谱纯化技术研究

发布时间：2018-05-04 16:19

本文选题：穿心莲内酯 + 分离纯化　；参考：《江苏农业科学》2017年21期

【摘要】：以穿心莲提取物为试验原料,采用动态轴向压缩制备色谱技术进行分离纯化,以制备高纯度穿心莲内酯。制备柱装填纯化材料:硅胶基质C1810μm,装填质量300 g,实测柱床规格50 mm×250 mm。以甲醇水为流动相进行纯化工艺优化,结果表明,甲醇水体系下水相添加0.05 mol/L乙酸铵能促进穿心莲内酯的溶解,该体系下穿心莲内酯最佳纯化工艺条件:流动相55%甲醇水+45%0.05 mol/L乙酸铵缓冲液,流速60.0 mL/min;检测波长254 nm,温度为室温。
[Abstract]:Andrographis andrographis extract was separated and purified by dynamic axial compression chromatography to prepare high purity andrographolide. The preparation of column packing and purification material was as follows: silica gel matrix C1810 渭 m, loading mass 300 g, and the measured column bed size 50 mm 脳 250 mm. The purification process was optimized with methanol-water as mobile phase. The results showed that the addition of 0.05 mol/L ammonium acetate to the aqueous phase of methanol water system could promote the dissolution of andrographis lactone. The optimum purification conditions of andrographolide in this system are as follows: the mobile phase is 55% methanol water 450.05 mol/L ammonium acetate buffer, the flow rate is 60.0 mL / min, the detection wavelength is 254 nm, the temperature is room temperature.
【作者单位】：江苏省泰州市粮油质量监测所;江苏大学食品与生物工程学院;
【基金】：江苏省自然科学基金青年基金(编号:20150500) 江苏大学高级人才科研启动基金(编号:15JDG070)
【分类号】：O657.72;R284.2

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