基于裸电极构建比率电化学生物传感器与药物分子检测研究

发布时间：2018-05-06 06:31

本文选题：电化学生物传感器 + 比率　；参考：《青岛大学》2017年硕士论文

【摘要】：近年来,由于人口老龄化日益严重、生态环境遭到破坏、不健康的生活方式等多方面因素的影响,肿瘤患者人数逐年增加。而肿瘤化疗也取得了相当大的突破,肿瘤患者的生存时间被大大延长。然而患者机体由于代谢不完全导致的药物残留形成的严重副作用也会因此对人体造成二次伤害。因此,药物治疗在一定程度上受到了限制。测定体液中残余药品,尤其是血液样品中药品的浓度对于用药安全的评估具有重要意义。电化学传感器因其具有灵敏度高、选择性好等优点,一直是分析科学中一个非常活跃的研究前沿。随着对传感器的结构构建过程的不断深入探究,比率电化学生物传感器越来越广泛地引起科研工作者的关注。本文基于比率电化学检测方法,构建电化学传感器用于抗肿瘤药物如盐酸阿霉素DOX和槲皮素Qu的检测:一、基于裸玻碳电极GCE检测抗肿瘤药物DOX的比率电化学生物传感器。利用DOX与亚甲基蓝MB电化学活性高的特性,将二者直接加入电解液中,而无需对电极进行任何修饰;DOX与GCE之间发生氧化还原,出现显著的电信号;而MB作为参比出现稳定的信号;在控制MB浓度不变的情况下,随着DOX加入量的增大,DOX引起的电信号逐渐增强;通过电化学工作站对DOX和MB的电信号进行检测,实现了对DOX的比率双重电信号快速和高灵敏检测。比率法可以明显降低源自环境和个人操作因素的电化学传感器的固有系统误差和偶然误差,从而显著增强实验方案的再现性和稳定性。二、基于比率检测方法,我们通过直接使用裸GCE作为信号传感平台构建了一个简易的电化学传感器来灵敏地检测电解液中的Qu。此外,硫堇TH被设为内部参考电化学探针。IQu/ITH与[Qu]之间具有良好的线性关系,同时检测限低至3.1 nM。在实际食品样品的干扰实验中,该比率检测方法对Qu具有高度的选择性和灵敏性。与之前报道的研究相比,本实验取得了更宽的检测范围(0-15μM),该研究方法的优点是Qu的电化学测定可以直接在电解液中进行,类似于在水性介质中被广泛认可的荧光传感方法。该方法操作简单,应用广泛,可以扩展到电解液中的其他类似比率电化学传感器的构建,这将有助于电化学传感器的进一步发展。
[Abstract]:In recent years, the number of cancer patients has increased year by year due to the aging of population, the destruction of ecological environment, unhealthy lifestyle and other factors. Tumor chemotherapy has also made a considerable breakthrough, tumor patients survival time has been greatly extended. However, severe side effects caused by incomplete metabolism can also cause secondary injury. Therefore, drug treatment is limited to some extent. It is important to determine the concentration of residual drugs in body fluid, especially in blood samples. Electrochemical sensors have been a very active research frontier in analytical science because of their high sensitivity and good selectivity. With the research on the structure of the sensor, the ratio of electrochemical biosensor has attracted more and more attention of researchers. Based on the ratio electrochemical detection method, an electrochemical sensor was constructed for the detection of antitumor drugs such as adriamycin hydrochloride (DOX) and quercetin qu. Firstly, a ratio electrochemical biosensor based on bare glassy carbon electrode (GCE) was used to detect the antitumor drug DOX. Based on the high electrochemical activity of DOX and methylene blue MB, they were directly added into the electrolyte without any modification of the electrode between dox and GCE. MB is used as a reference signal. When the concentration of MB is kept constant, the electrical signal caused by DOX and MB is gradually enhanced with the increase of the amount of DOX, and the electrical signals of DOX and MB are detected by electrochemical workstation. The rate dual signal detection of DOX is fast and highly sensitive. The ratio method can obviously reduce the inherent systematic error and accidental error of the electrochemical sensor derived from the environmental and personal operating factors, thus significantly enhancing the reproducibility and stability of the experimental scheme. Secondly, based on the ratio detection method, we constructed a simple electrochemical sensor by using naked GCE as the signal sensing platform to detect the Qui in electrolyte sensitively. In addition, thionine th has a good linear relationship with [qu] as an internal reference electrochemical probe. The detection limit is as low as 3.1 nm. The ratio detection method is highly selective and sensitive to qu in the interference test of real food samples. Compared with previous studies, a wider detection range of 0-15 渭 m ~ (-1) has been obtained. The advantage of this method is that the electrochemical determination of qu can be carried out directly in electrolyte, which is similar to the widely accepted fluorescence sensing method in aqueous media. The method is simple in operation and widely used, and can be extended to the construction of other similar ratio electrochemical sensors in electrolyte, which will be helpful to the further development of electrochemical sensors.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1;TP212.3;TQ460.72

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