纸板碳化电极提高生物电化学系统产电效率微生物机理研究

发布时间：2018-05-07 05:17

本文选题：纤维素纸板 + 碳化电极　；参考：《环境科学学报》2017年02期

【摘要】：采用Solexa高通量测序技术和蛋白定量方法,分析了碳化纤维素纸板制备的层状波纹碳(LCC)电极与石墨板(GP)电极在生物电化学系统运行过程形成的生物膜微生物群落结构和生物量差异.结果表明,LCC电极良好的生物电化学性能不仅取决于其良好的导电性能,还与其表面生物膜微生物群落结构有关;LCC电极表面生物膜微生物量高,且对产电微生物的富集效果好.LCC电极与GP电极表面生物膜分别得到16S rRNA基因V3区优化序列12643条和12837条,经97%相似度归并后获得的OTUs数量分别为2786和3130;α多样性分析显示,GP电极生物膜微生物多样性相对更丰富.Proteobacteria、Firmicutes和Bacteroidetes在两种电极生物膜中含量最为丰富,这3个门细菌序列数分别占总序列数的76%(LCC)和85%(GP).在属分类水平上,LCC电极生物膜由383个属的细菌构成,而GP电极生物膜则有456个属.深入分析电极生物膜微生物群落结构有助于进一步认识LCC电极提高生物电化学系统产电效率的机理.
[Abstract]:Solexa high-throughput sequencing technique and protein quantification method were used. The biofilm microbial community structure and biomass difference between laminated corrugated carbon dioxide (LCC) electrode and graphite plate GPCC electrode prepared by carbonized cellulose paperboard in the operation of bioelectrochemical system were analyzed. The results showed that the good bioelectrochemistry properties of LCC electrode not only depended on its good electrical conductivity, but also related to the structure of biofilm microbial community on the surface of LCC electrode and the high biomass of biofilm microorganism on the surface of LCC electrode. The optimal sequence of 16s rRNA gene V3 region was obtained from the biofilm on the surface of LCC electrode and GP electrode, respectively, and the optimized sequences were 12643 and 12837, respectively. The number of OTUs obtained by merging 97% similarity was 2786 and 3130, respectively, and 伪 diversity analysis showed that the microbial diversity of biofilm was relatively more abundant. Proteobacteriae Firmicutes and Bacteroidetes were the most abundant in the biofilm of the two electrodes. The number of these three bacteria accounted for 76% of the total number of sequences, and 85% of the total number of bacteria. The LCC electrode biofilm is composed of 383 genera of bacteria and 456 genera of GP electrode biofilm. Further analysis of the microbial community structure of the electrode biofilm is helpful to further understand the mechanism of the LCC electrode increasing the efficiency of the bioelectrochemical system.
【作者单位】：厦门理工学院环境科学与工程学院;中国科学院城市环境研究所;江西师范大学化工学院;
【基金】：福建省中青年教师教育科研项目(No.JA15372) 国家自然科学基金(No.51208490) 厦门理工学院高层次人才科技类项目(No.E2015028)~~
【分类号】：O646

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