基于细菌纤维素的毛细管电泳法同时测定果蔬中3种苯并咪唑类杀菌剂

发布时间：2018-05-08 04:42

  本文选题：毛细管电泳 + 细菌纤维素　； 参考：《色谱》2017年12期

【摘要】：建立了高效毛细管电泳同时测定果蔬中3种常见的苯并咪唑类杀菌剂(甲基硫菌灵、多菌灵和苯菌灵)的分析方法。以细菌纤维素(BC)为电泳缓冲液添加剂来提高毛细管电泳的分离能力。系统考察了检测波长、缓冲液离子强度、缓冲液pH、分离电压及BC添加量对3种苯并咪唑类杀菌剂分离效果的影响。最终的优化条件:H3BO3/Na2B4O7缓冲液(4 mmol/L,pH 9.0);BC添加质量分数0.3%;运行电压15 kV;分离柱温25℃;检测波长275 nm。3种苯并咪唑类杀菌剂在8 min之内可实现基线分离及准确定量。结果显示:3种苯并咪唑类杀菌剂在各自线性范围内线性关系良好,相关系数(r2)≥0.997;检出限为5.0~10.0μg/L;保留时间及峰面积的日间相对标准偏差(n=5)分别为0.82%~1.0%和2.4%~2.9%。该法用于葡萄、番茄及黄瓜中3种苯并咪唑类杀菌剂的测定,加标回收率为93.5%~103.0%,RSD≤8.0%。该法可作为果蔬中甲基硫菌灵、多菌灵和苯菌灵同时检测的有效手段。
[Abstract]:A method for simultaneous determination of benzimidazole fungicides (methyl thiamethyl, carbendazim and benzyl) in fruits and vegetables by high performance capillary electrophoresis (HPCE) was established. The separation ability of capillary electrophoresis was improved by using bacterial cellulose (BC) as buffer additive. The effects of detection wavelength, ionic strength of buffer, pH of buffer, separation voltage and addition of BC on the separation of three benzimidazole fungicides were systematically investigated. The optimized conditions were as follows: 4 mmol / L buffer solution of H _ 3BO _ 3 / Na _ 2B _ 4O _ 7, pH 9.0g / L, running voltage of 15 KV, column temperature of 25 鈩，

本文编号：1860030