荧光纳米复合材料的制备及在金属增强荧光和生物成像中的应用

发布时间：2018-05-09 01:08

本文选题：金属增强荧光效应 + RAFT聚合　；参考：《青岛大学》2016年硕士论文

【摘要】：在本文中我们合成并研究了两种纳米复合材料一种是pH敏型的银@聚合物复合材料的制备及其增强荧光效应的研究;另一种是氧化石墨烯包覆的四氧化三铁@多空二氧化硅(Fe3O4@mSiO2)复合材料用于磁控生物成像。刺激响应型“软”壳的金属荧光增强系统的制造是一种实现可调金属荧光增强行为的有效手段。在本篇论文中,不同聚合度的pH响应型的荧光体修饰的聚丙烯酸(PAA)通过可逆加成断裂链转移聚合被合成,选用罗丹明B修饰的RAFT试剂作为活性剂,随之通过Ag-S协调作用将其固定到银纳米粒子表面,形成了以纳米银为核心,以聚丙烯酸为壳的核-壳纳米复合材料。这种pH响应的银@聚合物核-壳纳米复合材料在药物输送、传感器和纳米器件方面具有潜在的应用。一种新型的氧化石墨烯包覆的Fe3O4@mSiO2纳米复合材料被合成用于生物成像。首先,通过水热法合成Fe3O4纳米粒子;其次,在Fe3O4表面修饰一层多孔二氧化硅壳,然后将罗丹明B负载到Fe3O4@mSiO2复合材料内;最后,将氧化石墨烯包裹在Fe3O4@mSiO2表面形成Fe3O4@mSiO2@GO纳米复合材料。氧化石墨烯的包覆可以有效地防止染料自释放。MTT毒性实验表明,罗丹明B负载的Fe3O4@mSiO2@GO复合材料在适宜的浓度呈现出很小的毒性并且可以在磁场的作用下定向移动。因此,这种磁性纳米复合材料在细胞成像方面有巨大的应用潜力。
[Abstract]:In this paper, we have synthesized and studied two kinds of nanocomposites, namely, the preparation of pH sensitive silver @ polymer composites and their enhanced fluorescence effect. The other is the Fe _ 2O _ 4 / Fe _ 3O _ 4 / m Sio _ 2 composite coated with graphene oxide. The fabrication of metal fluorescence enhancement system for "soft" shell is an effective way to realize the tunable metal fluorescence enhancement behavior. In this paper, the pH-responsive fluorescent modified polyacrylate PAA with different degree of polymerization was synthesized by reversible addition chain transfer polymerization. Rhodamine B modified RAFT reagent was used as the active agent. Then, the core-shell nanocomposites with nano-silver as the core and polyacrylic acid as the shell were formed by the coordinated action of Ag-S on the surface of silver nanoparticles. This pH-responsive silver @ polymer core-shell nanocomposite has potential applications in drug delivery, sensors and nanodevices. A novel Fe3O4@mSiO2 nanocomposite coated with graphene oxide has been synthesized for bioimaging. Firstly, Fe3O4 nanoparticles were synthesized by hydrothermal method; secondly, a porous silica shell was modified on the surface of Fe3O4, then Rhodamine B was loaded into Fe3O4@mSiO2 composites; finally, graphene oxide was encapsulated on the surface of Fe3O4@mSiO2 to form Fe3O4@mSiO2@GO nanocomposites. The coating of graphene oxide can effectively prevent the dye self-release. MTT toxicity test shows that the Rhodamine B supported Fe3O4@mSiO2@GO composite exhibits little toxicity at the appropriate concentration and can move in a directional direction under the action of magnetic field. Therefore, this magnetic nanocomposite has great potential in cell imaging.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O631.5;TB33

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