利用亚临界丁烷分离茶树鲜叶中的阿维菌素

发布时间：2018-05-09 14:12

  本文选题：亚临界丁烷 + 阿维菌素　； 参考：《分析化学》2017年06期

【摘要】：以茶树鲜叶为原料,利用亚临界丁烷流体分离鲜叶表面残留的阿维菌素。在料溶比为1∶10(m/V)的条件下,利用响应面法(RSM)的Box-Benhnken Design(BBD)设计实验,考察不同温度、时间、分离次数等条件对多酚氧化酶活性的影响,同时分析亚临界丁烷处理对茶树鲜叶生理特性的影响。结果表明,亚临界丁烷流体在保持茶叶物理结构和多酚氧化酶活性基本不变的情况下,可有效分离茶叶中的阿维菌素;通过Design Expert软件分析,得出分离阿维菌素的最优工艺参数为温度45℃,时间30 min,分离次数1次,料液比为1∶10(m/V),分离效率大于91%,多酚氧化酶相对活性为25.73%。通过扫描电镜观察,茶树鲜叶结构在丁烷处理前后无明显变化。本研究结果表明,亚临界丁烷流体可有效分离茶叶中的农药残留,为亚临界流体在去除天然植物农残的应用中提供了实验依据和技术支持。
[Abstract]:Fresh tea leaves were used to separate abamectin from fresh leaves by subcritical butane fluid. At the ratio of 1: 10 m / v), the experiment was designed by using the response surface method (RSM), and the effects of different temperature, time and separation times on the activity of polyphenol oxidase were investigated. The effects of subcritical butane treatment on the physiological characteristics of tea fresh leaves were also analyzed. The results showed that the subcritical butane fluid could effectively separate avermectin from tea leaves under the condition of keeping the physical structure of tea leaves and the activity of polyphenol oxidase unchanged, and could be analyzed by Design Expert software. The optimum technological parameters for separation of avermectin were as follows: temperature 45 鈩，

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