疏水改性海藻酸钠的载药释药性能及其与壳聚糖的协同絮凝作用

发布时间：2018-05-09 21:55

  本文选题：海藻酸钠 + 疏水改性　； 参考：《青岛科技大学》2016年硕士论文

【摘要】：海藻酸钠是一种天然高分子多糖,在自然界中广泛存在,具有良好的生物相容性、非免疫性、安全性及生物降解性,被广泛应用于生物医学等领域。本文以十二烷基缩水甘油醚为接枝原料对海藻酸钠进行疏水改性,得到了两亲性的海藻酸钠接枝产物。通过表面张力、动态光散射、Zeta电位等研究方法,系统研究了疏水改性海藻酸钠(HM-alginate)在水溶液中的胶束化行为,以及HM-alginate对疏水性物质的增溶能力和对药物的缓释性能,并对HM-alginate与壳聚糖的协同絮凝作用进行了研究。1.在碱性条件下,以十二烷基缩水甘油醚为疏水性接枝原料,对海藻酸钠进行疏水改性,得到了具有两亲性的海藻酸钠衍生物。所合成的疏水改性海藻酸钠具有明显的表面活性,在水溶液中可以形成以疏水性烷氧基链为内核和以亲水性海藻酸钠为外壳的核-壳结构的胶束,其临界胶束浓度为0.4 mg/m L。形成胶束时溶液的粒径在1000 nm左右,Zeta电位为-63±1 mV,该胶束在溶液中能够稳定存在。2.疏水改性海藻酸钠对脂溶性染料苏丹红Ⅳ有良好的增溶作用,通过将苏丹红Ⅳ包裹在胶束的疏水内核中增加其在溶液中的溶解度。3.疏水改性海藻酸钠对疏水药物氯法齐明和两性霉素B均有良好的增溶作用,可以使这两种药物在溶液中的溶解度分别增大1100倍和160倍。氯法齐明和两性霉素B在HM-alginate溶液中的溶解度随NaCl浓度的升高而降低,随溶液pH的增大而降低。4.疏水改性海藻酸钠对氯法齐明及两性霉素B均有较好的缓释作用,HM-alginate溶液浓度越大,缓释效果越明显。5.在疏水改性海藻酸钠溶液中加入壳聚糖后可以观察到明显的絮凝现象,疏水改性海藻酸钠与壳聚糖(HM-alginate/chitosan)的絮凝作用可以用来提取水中的疏水性染料苏丹红Ⅳ。HM-alginate/chitosan对苏丹红Ⅳ的提取率随HM-alginate浓度的增加而降低,随壳聚糖浓度的增加先升高后降低。pH为4.0时,1.0 mg/m L的HM-alginate和3.0 mg/mL的壳聚糖溶液混合后,对苏丹红Ⅳ的提取效果最好,提取率约为45.1%。6.疏水改性海藻酸钠与壳聚糖的絮凝作用受溶液pH值的影响较大,在pH值为3.0-3.8时,HM-alginate/chitosan对苏丹红Ⅳ的提取效果最好,提取率在75%左右。7.疏水改性海藻酸钠与壳聚糖的絮凝作用具有选择性,可以选择性除去溶液中的疏水染料苏丹红Ⅳ,而对亲水染料亚甲基蓝没有显著影响,且HM-alginate/chitosan对苏丹红Ⅳ的提取率不受溶液中亚甲基蓝存在的影响。在亚甲基蓝存在的条件下,pH值为3.0-3.8时HM-alginate/chitosan对苏丹红Ⅳ的提取率仍为75%左右,对亚甲基蓝的提取率在10%以下。
[Abstract]:Sodium alginate is a kind of natural polymer polysaccharide, which is widely existed in nature and has good biocompatibility, non-immunity, safety and biodegradability. It is widely used in biomedicine and other fields. The hydrophobic modification of sodium alginate with dodecyl glycidyl ether was carried out and the amphiphilic sodium alginate grafted product was obtained. The micellization behavior of hydrophobic modified sodium alginate (HM-alginate) in aqueous solution, the solubilization ability of HM-alginate to hydrophobic substances and the sustained release properties of drugs were systematically studied by means of surface tension, dynamic light scattering and Zeta potential. The synergistic flocculation of HM-alginate and chitosan was studied. The hydrophobic modification of sodium alginate with dodecyl glycidyl ether as hydrophobic graft material was carried out under alkaline conditions, and a amphiphilic sodium alginate derivative was obtained. The hydrophobic modified sodium alginate has obvious surface activity and can form core-shell micelles with hydrophobic alkoxyl chain as core and hydrophilic sodium alginate as shell in aqueous solution. The critical micelle concentration is 0.4 mg/m / L. The particle size of the solution was about 1000 nm and the Zeta potential was -63 卤1 MV. The micelle could exist stably in the solution. The hydrophobic modified sodium alginate has a good solubilization effect on the liposoluble dye Sudan 鈪，

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