多壁碳纳米管分散型固相萃取-高效液相色谱测定降糖保健食品中非法添加药物罗格列酮

发布时间：2018-05-11 09:18

本文选题：多壁碳纳米管 + 分散型固相萃取　；参考：《食品工业科技》2017年02期

【摘要】：本文建立多壁碳纳米管分散型固相萃取-高效液相色谱法测定降糖类保健食品中非法添加物罗格列酮的检测方法,重点考察了样品提取溶剂和提取时间、净化剂类型、组成、使用量和净化时间对回收率的影响。结果表明,样品前处理最优条件为:采用无水甲醇超声提取20 min,经多壁碳纳米管(MWCNTs)和N-丙基乙二胺(PSA)净化1 min。所建立的高效液相色谱法测定降糖类保健食品中罗格列酮的线性范围为1.0~50.0μg/m L,相关系数为0.9997,检出限和定量限分别是0.02μg/m L和0.06μg/m L。样品加标平均回收率为77.3%~93.2%,相对标准偏差(RSD)小于10%。该样品前处理方法简便、快速且成本较低,适用于批量保健食品中罗格列酮的快速检测。
[Abstract]:In this paper, a method for the determination of rosiglitazone, an illegal additive in hypoglycemic health food, was established by multi-walled carbon nanotube dispersion solid-phase extraction and HPLC. The extraction solvent and extraction time, the type and composition of purifying agent were investigated. The effect of usage and purification time on recovery rate. The results showed that the optimal pretreatment conditions were as follows: ultrasonic extraction with anhydrous methanol for 20 min and purification by MWCNTs and N- propyl ethylenediamine (PSAs) for 1 min. The linear range for determination of rosiglitazone in hypoglycemic health food by HPLC was 1.0 渭 g / mL, the correlation coefficient was 0.9997, the detection limit and quantitative limit were 0.02 渭 g / mL and 0.06 渭 g / mL, respectively. The average recovery was 77.3% and the relative standard deviation (RSD) was less than 10. The method is simple, rapid and low cost. It is suitable for rapid determination of rosiglitazone in batch health food.
【作者单位】：广东食品药品职业学院;广州市食品药品监督管理局审评认证中心;华南农业大学食品学院;
【基金】：国家自然科学基金项目(31401591) 广东食品药品职业学院院级科研项目(2014YZ004)
【分类号】：TS218;O657.72

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