介孔沸石材料负载传统基质用于激光解吸电离-飞行时间质谱分析小分子化合物

发布时间：2018-05-12 11:30

本文选题：介孔沸石 + 激光解吸电离-飞行时间质谱　；参考：《分析化学》2017年10期

【摘要】：考察了介孔沸石材料负载传统有机基质α-氰基-4-羟基桂皮酸(CHCA)用于基质辅助激光解吸电离-飞行时间质谱(MALDI-TOF-MS)分析多肽Substance P和氟喹诺酮类药物等小分子的效果。在相同的MALDI-TOF-MS质谱条件下,与传统CHCA进行了比较,同时分别考察了不同硅铝比(SiO_2/Al_2O_3)的ZSM-5以及不同介孔大小的Beta与ZSM-5沸石载体对Substance P的检测效果。结果表明,沸石负载CHCA新型复合基质具有抑制碱金属离子峰、消除干扰碎片离子、简化与改善质谱图、提高离子化效率等优点。实验结果表明,沸石表面酸性越强,有力介孔能够充分包裹CHCA分子,则复合基质抑制干扰碎片和提高离子化效率的能力越高。复合基质成功应用于复杂样品中恩诺沙星与诺氟沙星药物小分子的MALDI-TOF-MS检测。
[Abstract]:The effect of mesoporous zeolite loaded with traditional organic matrix 伪 -cyano-4-hydroxycinnamic acid (CHCA) on matrix assisted laser desorption ionization / time of flight mass spectrometry (MALDI-TOF-MS) analysis of polypeptide Substance P and fluoroquinolones was investigated. Under the same MALDI-TOF-MS mass spectrum conditions, compared with the traditional CHCA, the detection of Substance P by ZSM-5 with different Si-Al / SiO _ 2 / Al _ 2O _ 3 ratio and Beta and ZSM-5 zeolite carriers with different mesoporous sizes were investigated respectively. The results show that the new CHCA composite matrix supported by zeolite has the advantages of inhibiting alkali metal ion peak, eliminating interference of fragment ions, simplifying and improving the mass spectrogram and improving the ionization efficiency. The experimental results show that the stronger the acidity of zeolite surface, the more powerful mesoporous CHCA molecules can be encapsulated, the higher the ability of the composite matrix to suppress interference fragments and improve the ionization efficiency. The composite matrix was successfully applied to the detection of enrofloxacin and norfloxacin in complex samples by MALDI-TOF-MS.
【作者单位】：中国农业科学院农业质量标准与检测技术研究所农产品质量与食物安全重点开放实验室;
【基金】：中国农业科学院基本科研业务费专项(No.1610072016010);中国农业科学院科技创新工程项目资助~~
【分类号】：O657.63

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