复方板蓝根颗粒APCI-MS指纹图谱及化学成分的LC-APCI-MS~n研究

发布时间：2018-05-14 01:09

  本文选题：复方板蓝根颗粒 + 指纹图谱　； 参考：《质谱学报》2017年03期

【摘要】：建立了复方板蓝根颗粒提取物的大气压化学电离质谱(APCI-MS)的指纹图谱分析方法,并利用液相色谱-大气压化学电离-串联质谱(LC-APCI-MS~n)技术对主要指纹峰的归属进行确认。结果表明:质谱指纹图谱确定了10批不同来源复方板蓝根颗粒提取物的10个共有峰,各提取物的指纹图谱相似度均在97%以上。化学成分的LC-APCI-MS~n分析鉴别了复方板蓝根颗粒提取物中吲哚类、奎唑酮类、有机酸类和卟啉类等12个化合物,并确定了5个质谱指纹峰的归属。采用体外实验研究对复方板蓝根颗粒提取物的抗病毒活性进行评价,结果表明,其具有一定的抗病毒活性。该指纹图谱的精密度、稳定性和重现性良好,化学成分分析快捷、灵敏,结合化学成分分析和药效学研究,可为复方板蓝根颗粒质量控制标准的制订奠定基础,同时也为深入研究其药效物质基础和谱效关系提供理论依据。
[Abstract]:A fingerprint analysis method for APCI-MS of compound Isatis root granule extract was established. The main fingerprint peaks were confirmed by liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MSN) technique. The results showed that the 10 common peaks of 10 batches of compound Radix Isatidis granules extracts were identified by mass spectrometry, and the fingerprint similarity of each extract was more than 97%. LC-APCI-MS~n analysis of the chemical constituents identified 12 compounds such as indole, quezolidone, organic acids and porphyrins in compound Isatis root granule extracts, and determined the attribution of five MS fingerprint peaks. The antiviral activity of compound Radix Isatidis granules extract was evaluated in vitro. The results showed that it had certain antiviral activity. The fingerprint has good precision, stability and reproducibility, quick and sensitive chemical component analysis, combined with chemical composition analysis and pharmacodynamics study, can lay a foundation for the formulation of quality control standard of compound Isatis root granules. At the same time, it also provides the theoretical basis for the further study of the substance basis and spectrum effect relationship of its pharmacodynamics.
【作者单位】： 核工业北京地质研究院;中国科学院长春应用化学研究所;
【分类号】：O657.63;R286.0
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本文编号：1885639