基于闭合型双极化电极原理的电化学研究和活体分析应用

发布时间：2018-05-14 16:47

  本文选题：闭合型双极化电极 + 多巴胺　； 参考：《湘潭大学》2016年硕士论文

【摘要】：双极化电化学是以双极化电极(BPE)为工作电极,电极的两端分别发生氧化或还原反应的一种电化学研究方法,它包括开放型双极化体系和闭合型双极化体系两种类型。本论文主要研究的是闭合型双极化体系,基于闭合型双极化原理,我们为鼠脑中一些神经递质或活性分子(如多巴胺、抗坏血酸)的电化学检测提供了一种新型的有效检测方法。具体工作概括如下:(1)我们首先研究了闭合型双极化体系中电极尺寸和电极两端物质的浓度对半波电位的影响,然后选择一个最合适的K_3Fe(CN)_6浓度和电极尺寸制备了K_3Fe(CN)_6溶液填充的圆柱形碳纤维电极(CFEs),用于原位检测鼠脑中的多巴胺(DA)。并比较了该电极与传统的填充KCl或Ru(NH3)6Cl3溶液的电极稳定性,灵敏度和半波电位的大小,最后证明我们所制备的电极对活体中儿茶酚胺的检测具有高稳定性、高灵敏度和对动物的低损害性等优点。(2)我们对传统意义上的闭合型双极化电极装置进行了一定的改装,即撤去外界所施加的电压,在电极两端分别加入可以发生氧化或还原反应的电化学物质,利用电极两端自发地发生氧化或还原反应产生的电势差,将该体系改装成一个可自行发电的原电池。电压和功率经两根理想型非极化电极(闭合型双极化体系中的驱动电极)进行输出。在此设计下,我们研究了H_2-O_2,K_4Fe(CN)_6-O_2,K_3Fe(CN)_6-Fc(COOH)2,K_3Fe(CN)_6-DA四种电池的工作性能,发现功率和电压均有较好地输出。(3)基于改装后的原电池原理的闭合型双极化体系,我们制备了一种检测活体中抗坏血酸释放的微型生物传感器。即在碳纤维电极内填充含有支持电解质的一定浓度的K_3Fe(CN)_6溶液,并在电极表面修饰多壁碳纳米管,将其作为工作电极用于活体中抗坏血酸的原位检测。我们对该基于原电池原理所制备的生物传感器性能进行了研究,发现利用此方法可以实现对鼠脑中抗坏血酸的高稳定、高选择性检测。
[Abstract]:Bipolar electrochemistry is a kind of electrochemical research method in which bipolarization electrode BPEE is used as working electrode. It includes two kinds of open bipolarization system and closed bipolarization system, which have redox or reduction reaction at the two ends of the electrode respectively. In this paper, we mainly study the closed bipolarization system. Based on the closed bipolarization principle, we are some neurotransmitters or active molecules in the brain, such as dopamine, dopamine. The electrochemical detection of ascorbic acid provides a new and effective method for detection of ascorbic acid. The specific work is summarized as follows: (1) We first studied the effects of electrode size and the concentration of matter at both ends of the electrode on the half-wave potential in a closed bipolar system. Then the cylindrical carbon fiber electrode filled with K_3Fe(CN)_6 solution was prepared by selecting the most suitable K_3Fe(CN)_6 concentration and electrode size for in situ detection of dopamine dadamine in the brain. The stability, sensitivity and half-wave potential of the electrode were compared with those of the conventional solution filled with KCl or Ru(NH3)6Cl3. Finally, it was proved that the prepared electrode had high stability for the detection of catecholamine in vivo. The advantages of high sensitivity and low damage to animals.) We have modified the closed bipolar electrode device in the traditional sense, that is, to remove the external applied voltage. The electrochemical materials which can be oxidized or reduced were added at the two ends of the electrode, and the potential difference between the two ends of the electrode was used to convert the system into a self-generated primary battery. The voltage and power are outputted by two ideal non-polarized electrodes (driving electrodes in closed bipolar systems). Under this design, we have studied the performance of HStut _ 2-O _ 2C _ S _ 4FeC _ S _ 4C _ S _ 4 C _ S _ 6O _ 2C _ T _ 6-O _ 2C _ T _ 3F _ C _ C _ C _ C _ C _ C _ C _ C _ C _ C _ T _ (6), and found that the output of power and voltage is better. 3) A closed double polarization system based on the modified principle of the primary cell. We have prepared a micro biosensor for the detection of ascorbic acid release in vivo. The carbon fiber electrode was filled with a certain concentration of K_3Fe(CN)_6 solution containing supporting electrolyte, and the multi-walled carbon nanotubes were modified on the electrode surface, which was used as the working electrode for the in-situ detection of ascorbic acid in vivo. We have studied the performance of the biosensor based on the principle of primary battery and found that this method can be used to detect ascorbic acid in brain with high stability and selectivity.
【学位授予单位】：湘潭大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.1
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本文编号：1888693