固相萃取-气相色谱-质谱法测定植物饮料中的9种植物毒素

发布时间：2018-05-17 00:19

本文选题：植物饮料 + 植物毒素　；参考：《现代食品科技》2017年03期

【摘要】：建立了固相萃取(SPE)净化结合气相色谱-质谱联用(GC-MS)同时检测植物饮料中9种植物毒素(侧柏酮、龙蒿脑、长叶薄荷酮、黄樟素、甲基丁香酚、香豆素、α-细辛脑、β-细辛脑和山道年)的分析方法。样品高速离心后,采用HLB固相萃取小柱富集净化,洗脱液经氮吹浓缩,以DB-5MS色谱柱分离,GC-MS选择离子监测模式测定。实验表明,9种植物毒素在5~1000μg/L浓度范围内线性关系良好,相关系数均大于0.999;方法检出限(S/N=3)为0.3~1.4μg/kg,定量限(S/N=10)为1.0~4.7μg/kg;阴性样品的3个添加水平的平均回收率在76.3%~99.7%之间,相对标准偏差(RSD,n=6)小于8.7%。该方法处理简单,准确高效,适用于植物饮料中9种植物毒素的检测。8个实际样品的检测结果显示,有1个草本饮料样品检出0.7 mg/kg甲基丁香酚。
[Abstract]:A method for the simultaneous determination of nine plant toxins (Platycladone, Artemisia artemisia, menthol, safrole, methyleugenol) in plant beverages by solid-phase extraction (SPE) purification and gas chromatography-mass spectrometry (GC-MS) was established. Methods of analysis of coumarin, 伪-asarone, 尾-asarone and mountain path years. After high-speed centrifugation, the samples were enriched and purified by HLB solid-phase extraction column, the eluate was concentrated by nitrogen blowing, and the ion monitoring mode was selected by DB-5MS chromatographic column separation GC-MS. The results showed that there was a good linearity in the concentration range of 5 ~ 1000 渭 g / L for nine plant toxins, and the correlation coefficients were all greater than 0.999.Methods the detection limit S / N ~ (3) was 0.3N ~ (-1) 渭 g 路kg ~ (-1), the quantification limit was 1.0 ~ 4.7 渭 g 路kg ~ (-1), and the average recovery of the three additive levels for negative samples was between 76.3% and 99.7%, and the detection limit was 0.31 渭 g 路kg ~ (-1), and the quantitative limit was 1.0 渭 g 路kg ~ (-1). The relative standard deviation (RSD) is less than 8. 7%. The method is simple, accurate and efficient, and can be used for the detection of 9 plant toxins in plant beverages. The results of 8 actual samples showed that one herb beverage sample detected 0.7 mg/kg methyleugenol.
【作者单位】：广州质量监督检测研究院;广东食品药品职业学院;华南理工大学轻工与食品学院;广东工业大学轻工化工学院;
【基金】：国家质检总局科技计划项目(2014QK052,2015QK161)
【分类号】：TS255.44;O657.63

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