HPLC法测定不同产地荆芥中6种黄酮类成分

发布时间：2018-05-18 03:07

本文选题：荆芥 + HPLC　；参考：《中草药》2017年11期

【摘要】：目的通过对不同产地荆芥Schizonepeta tenuifolia中6种黄酮类成分的检测,为其质量控制提供依据。方法采用高效液相色谱(HPLC)法建立荆芥药材中黄酮类成分的检测方法,并以6种黄酮类成分的量为数据来源,对其进行聚类分析。结果不同产地荆芥中6种黄酮类成分的量差异较大,木犀草苷、槲皮苷、橙皮苷、木犀草素、芹菜素、香叶木素的线性范围分别是0.030~0.148μg、0.222~1.108μg、0.357~1.784μg、0.058~0.292μg、0.054~0.269μg、0.050~0.247μg,加样回收率为96.32%~99.97%,RSD2.20%;其中,安徽产地聚为一类,河南1与广东产地为聚一类,河南2、河北、云南、四川、湖北、甘肃产地聚为一类。结论建立的方法简单、灵敏,可用于荆芥的多指标成分的质量控制;其次,河南1、河南2、河北、云南、四川、湖北、广东、甘肃的亲缘关系较近,可以作为同一药用来源。
[Abstract]:Objective to detect six flavonoids in Schizonepeta tenuifolia of Schizonepeta thaliana from different producing areas and to provide basis for its quality control. Methods High performance liquid chromatography (HPLC) was used to establish a method for the determination of flavonoids in Herba Schizonepeta, and the contents of 6 flavonoids were used as data source for cluster analysis. Results there were significant differences in the contents of six flavonoids in Schizonepeta from different habitats. The linear range of luteolin, quercetin, hesperidin, luteolin, apigenin and balsam lignin was 0.0300.148 渭 g / g 0.222n 1.108 渭 g / g 0.35784 渭 g 0.0580.292 渭 g 0.0540.269 渭 g / 0.269 渭 g / 渭 g / g, and the recovery was 96.3299.977.The concentration of RSD2.20 was the same as that in Anhui province. Henan 1 is clustered with Guangdong, Henan 2, Hebei, Yunnan, Sichuan, Hubei and Gansu. Conclusion the established method is simple and sensitive, and can be used for the quality control of multiple index components of Schizonepeta thaliana. Secondly, Henan 1, Henan 2, Hebei, Yunnan, Sichuan, Hubei, Guangdong and Gansu are close relatives, which can be used as the same medicinal source.
【作者单位】：辽宁中医药大学药学院;辽宁省组分中药工程技术研究中心;辽宁省现代中药研究工程实验室;
【基金】：“十二五”重大新药创制科技重大专项(2013ZX09507005-005) 辽宁省教育厅一般项目(L201605,L201608)
【分类号】：O657.72;R284.1

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