分子印迹固相萃

发布时间：2018-05-18 03:37

本文选题：流动注射 + 化学发光　；参考：《重庆师范大学》2017年硕士论文

【摘要】：流动注射化学发光是一种利用体系内被检测的物质的浓度,与化学发光强度在一定环境中呈一定的线性关系,而建立起来的一种在线的测定方法,其特点是灵敏度很高,检测快速,操作简单。但是其对物质的检测没有特异性,难以对复杂的混合物中的物质进行测定,所以,研究一种对物质特异性识别的检测方法就受到了广泛的关注。分子印迹法是利用目标分子合成的一种对目标分子有特异性识别的印迹聚合物,将此印迹柱与流动注射化学发光分析仪连用,就能精确的测定出目标分子,从而弥补了流动注射化学发光分析仪无特异性识别的缺点,由此建立起来的分子印迹流动注射化学发光分析方法特异性识别强,灵敏度高,操作简单,应用范围广。本文主要研究了核苷、磺胺类药物对在酸性条件下的高锰酸钾-甲醛发光体系的增强现象,并建立了对核苷、磺胺类物质检测的体系,以及各项优化参数。具体介绍如下:1.在酸性条件下,利用KMnO_4-HCHO发光体系,结合流动注射化学发光分析仪,对尿嘧啶核苷进行了测定,结果显示回归方程:ΔI=86.58×105C(mol/L)+0.6,相关系数为R=0.9955,检出限是8×10-6 mol/L,对浓度为1×10-3 mol/L的尿嘧啶核苷平行测定11次,相对标准偏差为4.3%,运用此方法对尿嘧啶核苷成功进行了检测。2.将流动注射系统与化学发光联用来测定鸟嘌呤核苷,可以快速的测定出其含量,实验所得其线性方程:ΔI=49.67×105C(mol/L)-302.77,相关系数为R=0.9993,检出限是1.5×10-7 mol/L,对浓度为1×10-6 mol/L的鸟嘌呤核苷平行测定11次,相对标准偏差为2.7%。3.胞嘧啶核苷为目标分子,合成对其有专一性识别的分子印迹聚合物(MIP),在酸性条件下,利用HCl-KMnO_4-HCHO发光体系,结合流动注射化学发光(FI-CL)分析方法,创建对胞嘧啶核苷测定有特异性识别的MIP-FI-CL分析方法,其线性范围是8×10-5mol/L-1×10-3mol/L,检出限是4×10-5mol/L,利用此方法测定复杂样品中的胞嘧啶核苷的含量,结果令人满意。4.在酸性条件下,利用HCl-KMnO_4-HCHO发光体系,结合流动注射化学发光(FI-CL)分析方法,以磺胺二甲基嘧啶为目标分子,合成对其有专一性识别的分子印迹聚合物(MIP),创建对磺胺二甲基嘧啶测定有特异性识别的MIP-FI-CL分析方法,其线性范围是1×10-4 mol/L-1.5×10-3 mol/L,检出限是6×10-5 mol/L,利用此方法测定复杂样品中的磺胺二甲基嘧啶的含量,结果令人满意。
[Abstract]:Flow injection chemiluminescence (FIA) is a kind of on-line determination method, which utilizes the concentration of the substance detected in the system and has a certain linear relationship with the chemiluminescence intensity in a certain environment. Rapid detection, simple operation. However, the detection of substances is not specific, so it is difficult to determine the substances in complex mixtures. Therefore, the study of a detection method for the specific identification of substances has been paid more and more attention. Molecular imprinting is a kind of imprinted polymer synthesized by target molecule, which can be used in combination with flow injection chemiluminescence analyzer to determine the target molecule accurately. Thus, the shortcomings of flow injection chemiluminescence analyzer without specific recognition were remedied, and the molecular imprinted flow injection chemiluminescence analysis method was established with strong specificity, high sensitivity, simple operation and wide application range. In this paper, the enhancement of nucleoside and sulfonamides on the luminescent system of potassium permanganate and formaldehyde under acidic conditions was studied, and the system for the detection of nucleoside and sulfanilamides was established, as well as the optimized parameters. The details are as follows: 1. Under acidic conditions, the uracil nucleoside was determined by using KMnO_4-HCHO luminescence system and flow injection chemiluminescence analyzer. The results showed that the regression equation was: 螖 Ion 86.58 脳 105Cnmol / L) 0.6, the correlation coefficient was RX 0.9955, the detection limit was 8 脳 10-6 mol / L, 11 times for uracil with concentration of 1 脳 10-3 mol/L, and the relative standard deviation was 4.30.The method was used to detect uracil successfully. The flow injection system and chemiluminescence were used to determine the content of guanine nucleoside. The linear equation was obtained as follows: 螖 Ion 49.67 脳 10 ~ (-5) Con mol / L ~ (-302.77), the correlation coefficient is R _ (0.9993), the detection limit is 1.5 脳 10 ~ (-7) mol / L, and the parallel determination of guanine nucleoside with concentration of 1 脳 10 ~ (-6) mol/L is 11 times. The relative standard deviation is 2.7. 3. Cytosine nucleoside was used as the target molecule to synthesize the molecularly imprinted polymer (MIP) with specific recognition. In acidic conditions, HCl-KMnO_4-HCHO luminescence system was used in combination with flow injection chemiluminescence (FI-CLL) analysis method. A MIP-FI-CL method for the determination of cytosine nucleoside was established. The linear range was 8 脳 10-5mol/L-1 脳 10 -3 mol / L and the detection limit was 4 脳 10 ~ (-5) mol / L. the method was used to determine the content of cytosine nucleoside in complex samples with satisfactory results. In acidic conditions, sulfadimethazine was used as the target molecule, using HCl-KMnO_4-HCHO luminescence system and flow injection chemiluminescence (FI-CLL) analysis method. The molecularly imprinted polymer (MIP) was synthesized and a MIP-FI-CL method for the determination of sulfadimethylpyrimidine was established. The linear range is 1 脳 10 ~ (-4) mol/L-1.5 脳 10 ~ (-3) mol / L and the detection limit is 6 脳 10 ~ (-5) mol 路L ~ (-1). The method is used to determine the content of sulfadimethylpyrimidine in complex samples with satisfactory results.
【学位授予单位】：重庆师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TQ460.72

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