氧化石墨烯选择性抗菌性能研究

发布时间：2018-05-19 16:29

  本文选题：氧化石墨烯 + 横向尺寸　； 参考：《武汉大学学报(理学版)》2017年04期

【摘要】：采用改进的Hummer方法合成氧化石墨烯,通过控制超声时间获得不同横向尺寸的纳米片,将其与大肠杆菌作用,发现氧化石墨烯的抗菌活性与其横向尺寸呈正相关,当氧化石墨烯平均横向尺寸为2.5μm时,大肠杆菌的失活率达到96.4%.以革兰氏阴性菌大肠杆菌、绿脓杆菌和革兰氏阳性菌金黄色葡萄球菌作为研究对象,考察氧化石墨烯的抗菌性能.结果表明,低浓度(≤100μg·mL~(-1))时氧化石墨烯对革兰氏阴性菌的抑制作用强于对革兰氏阳性菌的抑制作用,当浓度达到200μg·mL~(-1)时,3种细菌失活率均能达到90%以上.最后通过原子力显微镜(AFM)观察大肠杆菌在氧化石墨烯作用下随时间的变化,发现大肠杆菌被氧化石墨烯纳米片包裹,其抗菌作用推测是通过氧化石墨烯片的氧化应激和阻隔营养物质的输送实现.
[Abstract]:Graphene oxide was synthesized by improved Hummer method. Different transverse sizes of graphene were obtained by controlling ultrasonic time. It was found that the antibacterial activity of graphene oxide was positively correlated with the transverse size of Escherichia coli. When the average transverse size of graphene oxide was 2.5 渭 m, the inactivation rate of E. The antibacterial properties of graphene oxide were studied with Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus. The results showed that the inhibitory effect of graphene oxide on Gram-negative bacteria was stronger than that on Gram-positive bacteria at low concentration (鈮，

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