含偶氮苯侧链的水溶性共轭聚合物的合成、表征及应用

发布时间：2018-05-21 09:44

本文选题：水溶性共轭聚合物 + 乏氧检测　；参考：《南京邮电大学》2016年硕士论文

【摘要】：近年来,世界各国科学家合成了大量的水溶性共轭聚合物,并应用于生物检测。水溶性共轭聚合物(WSCPs)在结构上主要由两部分组成:(i)π-共轭主链,决定了共轭聚合物的主要光物理性质,如吸收和发射光谱,光捕捉能力和量子产率等。(ii)侧链亲水基团,如阳离子季铵盐,阴离子羧基盐,磺酸盐,以及多羟基化合物等亲水基团。基于这样的研究背景,同时结合了乏氧环境细胞检测的发展,我们设计合成了两种水溶性共轭聚合物—PF4(含聚乙二醇水溶性侧链),PF6(含季铵盐阳离子水溶性侧链),以蓝色荧光的聚芴为共轭主链并通过活性自由基聚合(ATRP)的方式在聚合物的侧链接上偶氮苯基团(偶氮苯是一种乏氧敏感基团和荧光淬灭剂,在乏氧条件下,偶氮苯被活化的偶氮还原酶打断,从而失去对荧光的淬灭能力,使材料的荧光恢复)。通过核磁共振光谱(1H NMR),紫外吸收(UV Spectra)和荧光发射(Fluorescence Spectra)的表征,证明了化合物的成功合成。之后,我们对材料的基本光物理性质,光学稳定性,自组装行为,荧光猝灭机理,在还原酶存在下的材料荧光恢复情况以及乏氧环境下材料对细胞成像效果进行了探究。实验数据证明我们设计合成的水溶性共轭聚合物探针材料具有较低的细胞毒性(MTT法)并且能够对乏氧环境下的细胞有良好的成像效果。
[Abstract]:In recent years, scientists around the world have synthesized a large number of water-soluble conjugated polymers and used in biological detection. The water-soluble conjugated polymer (WSCPs) is composed of two parts, the 蟺 -conjugate main chain, which determines the main photophysical properties of the conjugated polymer, such as absorption and emission spectra, phototrapping ability and quantum yield, etc., and the hydrophilic group of the side chain of the conjugated polymer. Hydrophilic groups such as cationic quaternary ammonium salts, anionic carboxyl salts, sulfonates, and polyhydroxyl compounds. Based on this research background, combined with the development of hypoxia environment cell detection, We have designed and synthesized two kinds of water-soluble conjugated polymer -PF4 (water-soluble side chain containing polyethylene glycol) (water-soluble side chain containing quaternary ammonium salt, using blue fluorescent polyfluorene as conjugate main chain and polymerization of ATRPthrough active free radical). Azophenyl groups on side links of polymers (azobenzene is an oxygen-sensitive group and fluorescent quencher, Under the condition of hypoxia, azobenzene was interrupted by the activated azo reductase, thus the quenching ability of the fluorescence was lost and the fluorescence of the material recovered. The successful synthesis of the compounds was confirmed by 1H NMR, UV Spectrag and fluorescence emission spectra. After that, we studied the basic photophysical properties, optical stability, self-assembly behavior, fluorescence quenching mechanism, fluorescence recovery in the presence of reductase and the effect of cell imaging in hypoxic environment. The experimental data show that the water-soluble conjugated polymer probe material we designed and synthesized has low cytotoxicity and has good imaging effect on cells in hypoxic environment.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O631;O657.3

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