硕桦化学成分的研究

发布时间：2018-05-24 09:42

本文选题：硕桦 + 桦木属植物　；参考：《吉林大学》2017年硕士论文

【摘要】：硕桦(Betula costata Trautv.)为桦木科桦木属的植物,其树皮具有清热解毒、止咳、平喘、消炎、杀菌等功效,用于治疗慢性痢疾、血尿以及粉刺、雀斑等疾病。本文从硕桦树皮的85%乙醇提取物中分离得到9个单体化合物(分别标记为SH-1~SH-9),经核磁、红外光谱等数据分析,结合理化性质,鉴定它们分别是白桦脂醇(SH-1),羽扇豆醇(SH-2),3-O-乙酰齐墩果酸(SH-3),桦木酮酸(SH-4),桦木酮(SH-5),17α-Hydroxy-28-nor-18α(H)-lup-20(29)-en-3-one(SH-6),苦杏仁苷(SH-7),槭木素E(SH-8)和16-羟基-17-甲氧基-槭木素E(SH-9)。其中,17α-Hydroxy-28-nor-18α(H)-lup-20(29)-en-3-one为首次从天然产物中分离得到的化合物,16-羟基-17-甲氧基-槭木素E和苦杏仁苷为首次从硕桦中分离得到的化合物。在化学成分的研究基础上,本论文首次建立了两种三萜类化合物硕桦树皮中羽扇豆醇(化合物SH-2)和3-O-乙酰齐墩果酸(化合物SH-3)含量的HPLC测定方法。首先,对提取溶剂、提取方法、提取时间以及液料比等影响因素进行了考察,确立了最佳的提取方法。其次,进行了方法学考察。实验结果表明,化合物SH-2和SH-3分别在0.08-2.50 mg/mL和0.16-5.00 mg/mL范围内峰面积(Y)和进样浓度(X)呈良好的线性关系,化合物SH-2和SH-3分析方法的日内精密度、日间精密度、重复性、稳定性、加样回收率的RSD均小于2%,说明所建立的含量测定方法具有精密度好、准确度高等优点。含量测定结果,采自新宾县的硕桦中SH-2的含量为1.83 mg/g,SH-3含量为5.25 mg/g,采自靖宇县的硕桦中SH-2的含量为0.14 mg/g,SH-3含量为4.66mg/g,采自靖宇县的白桦SH-2的含量为1.15 mg/g,SH-3含量为1.25 mg/g。表明不仅同属植物硕桦和白桦之间SH-2、SH-3化合物含量差异较大,而且不同产地的硕桦之间含量差别也很大。综上,在国内外学者研究的基础上,本文从硕桦皮乙醇提取物中共计分离鉴定了9个化合物,其中有一个为首次从天然产物中分离得到的化合物,2个为首次从硕桦中分得的化合物;同时还建立了硕桦皮中两种五环三萜类化合物的HPLC-DAD含量测定方法。本文的研究结果为建立硕桦的质量标准以及硕桦的开发利用提供了新的科学依据。
[Abstract]:Betula costata Trautv. It is a kind of birch plant in the family Betulaceae. Its bark is used to treat chronic dysentery, hematuria, acne, freckle and other diseases with antipyretic, antitussive, antiasthmatic, anti-inflammatory and bactericidal effects. In this paper, nine monomers (labeled as SH-1 and SH-9) were isolated from 85% ethanol extract from the bark of birch bark. The compounds were analyzed by NMR, IR and physicochemical properties. They were identified as Bai Hua aliphatic alcohol (SH-1), Lupanolol (SH-2) -acetyl oleanolic acid (SH-3), betulinic acid (SH-4), betulinone (17 伪 -Hydroxy-28-nor-18 伪) H-lup-2029-en-3-one (SH-6), amygdalin (SH-7), maple (ESH-8) and 16-hydroxy-17-methoxy (ESH-9). Among these compounds, 17 伪 -Hydroxy-28-nor-18 伪 -Han-lup-2029-en-3-one was isolated from natural products for the first time, and the compounds of 16-hydroxy-17-methoxy-maple lignin E and amygdalin were first isolated from birch. Based on the study of chemical constituents, a HPLC method for the determination of the contents of lupinol (SH-2) and 3-O- acetyl oleanolic acid (compound SH-3) in the bark of two triterpenoids was established for the first time. Firstly, the influence factors such as solvent, extraction method, extraction time and the ratio of liquid to material were investigated, and the best extraction method was established. Secondly, the methodology is investigated. The experimental results showed that the peak area of SH-2 and SH-3 were linear in the range of 0.08-2.50 mg/mL and 0.16-5.00 mg/mL, respectively. The precision, repeatability and stability of compound SH-2 and SH-3 were obtained. The RSD of the recoveries was less than 2, which indicated that the established method had the advantages of good precision and high accuracy. The results showed that the content of SH-2 was 1.83 mg / g SH-3 was 5.25 mg / g, the content of SH-2 was 0.14 mg / g SH-3 was 4.66 mg / g, the content of Bai Hua SH-2 was 1.15 mg / g SH-3 in Jingyu county. The results showed that not only the content of SH-2N SH-3 was different between the same genus Betula platyphylla and Bai Hua, but also the content of SH-2SH-3 was different among different habitats. In summary, based on the research of domestic and foreign scholars, nine compounds were isolated and identified from the ethanol extracts of Betula platyphylla. One of the compounds was isolated from natural products for the first time and two were isolated from Betula platyphylla for the first time. A HPLC-DAD method for the determination of two pentacyclic triterpenoids in Betula platyphylla bark was also established. The results of this paper provide a new scientific basis for the establishment of quality standards and the development and utilization of birch.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O629

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