UPLC法同时测定不同银黄制剂中10种成分

发布时间：2018-05-24 21:56

  本文选题：超高效液相色谱法 + 银黄制剂　； 参考：《中草药》2017年03期

【摘要】：目的建立同时测定银黄制剂中10种成分(新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素)的超高效液相色谱(UPLC)方法。方法采用Acquiyt UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.4%磷酸水溶液为流动相,梯度洗脱,体积流量0.4 m L/min,在326 nm波长处进行检测,柱温40℃,进样量1.0μL。结果银黄制剂中10种成分在考察线性范围内线性关系良好(r≥0.999 6),加样回收率均在97.43%~99.94%,RSD均小于2.0%。11批银黄制剂样品中新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素质量分数分别在0.236~3.419、5.279~26.220、0.495~4.714、0.130~2.702、0.310~3.210、0.363~5.036、35.209~133.289、1.493~6.635、1.546~5.393、0.254~0.823 mg/g。结论该方法简便,准确,快速,分离效果好,重复性好,可用于银黄制剂的质量控制,并为将来该制剂质量标准提升提供参考。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of 10 components (new Lv Yuan acid, cryptonic acid, isoLv Yuan acid A, isoLv Yuan acid B, isoLv Yuan acid C, baicalin, Chinese baicalin) in Yinhuang preparation. Methods Acquiyt UPLC BEH Che _ (18) column was used to determine the concentration of acetonitrile-0.4% phosphoric acid solution as mobile phase, and the volume flow rate was 0.4 mL / min. The column temperature was 40 鈩，

本文编号：1930729