纳米标记免疫层析法在农药残留检测中的应用研究进展

发布时间：2018-05-25 23:20

本文选题：农药残留 + 免疫层析技术　；参考：《农药学学报》2017年04期

【摘要】：农药残留超标已成为影响农产品质量安全的重要问题,迫切需要探寻开发灵敏、准确、可靠、便捷且适用性强的农药残留快速检测方法。免疫层析法是将抗原抗体特异性免疫反应和色谱层析分离技术相结合的一种快速检测方法,其中,基于胶体金标记的免疫层析技术以其便捷、成本低、可视化等优点而受到普遍欢迎。近年来随着量子点、时间分辨荧光微球、上转换发光纳米粒子等新型纳米标记材料的出现,免疫层析技术得到了广泛发展。文章从标记类型(非共价作用标记及共价作用标记)及标记材料(胶体金、纳米碳、量子点、上转换发光纳米粒子、磁性纳米颗粒、时间分辨荧光微球及荧光乳胶颗粒)等方面,综述了不同纳米材料标记的免疫层析技术及其在农药残留检测领域的研究及应用进展,可为深入开展农药残留免疫层析技术研究提供参考。
[Abstract]:Pesticide residue exceeding the standard has become an important problem affecting the quality and safety of agricultural products. It is urgent to explore and develop a sensitive, accurate, reliable, convenient and applicable rapid detection method for pesticide residues. Immunochromatography is a rapid detection method which combines antigen and antibody specific immune reaction with chromatographic chromatography separation technology. The immunochromatographic technique based on colloidal gold labeling is convenient and low cost. The advantages of visualization are generally welcomed. In recent years, with the appearance of quantum dots, time-resolved fluorescent microspheres, up-conversion luminescent nanoparticles and other new nano-labeling materials, immunochromatography technology has been widely developed. In this paper, labeling types (non-covalent labeling and covalent labeling) and labeling materials (colloidal gold, nano-carbon, quantum dots, up-conversion luminescent nanoparticles, magnetic nanoparticles), Time-resolved fluorescent microspheres and fluorescent latex particles were reviewed in this paper. The immunochromatographic techniques of different nanomaterials and their applications in the field of pesticide residue detection were reviewed. It can provide a reference for the further study of pesticide residue immunochromatography.
【作者单位】：浙江大学农药与环境毒理研究所;
【基金】：国家自然科学基金(31401768) 浙江省公益技术应用研究项目(2016C32004) 国家重点研发计划项目(2016YFD0201302)
【分类号】：O657.3;TQ450.263

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